Document Detail

Dedifferentiation of human articular chondrocytes is associated with alterations in expression patterns of GDF-5 and its receptors.
MedLine Citation:
PMID:  19874419     Owner:  NLM     Status:  In-Process    
Human articular chondrocytes are expanded in monolayer culture in order to obtain sufficient cells for matrix-associated cartilage transplantation. During this proliferation process, the cells change their shape as well as their expression profile. These changes resemble those that occur during embryogenesis, when the limb anlagen form the interzone that later develops the joint cleft. We analysed the expression profile of genes that are reportedly important for these changes during embryogenesis within the dedifferentiation process of adult articular chondrocytes. We found GDF-5, BMPR-Ib and connexin 43 up-regulated, as well as a down-regulation of BMPR-Ia and noggin. Connexin 32 could not be detected in either native cartilage or in dedifferentiated cells. The newly synthesized proteins were detected by immunofluorescence. There is evidence from our results that dedifferentiated chondrocytes resemble the cells from the interzone in developing synovial joints.
Werner Schlegel; Christian Albrecht; Peter Eckl; Harald Freudenthaler; Angelika Berger; Vilmos Vécsei; Stefan Marlovits
Related Documents :
18932239 - Altered gene expression in early osteochondrosis lesions.
9704639 - Antisense inhibition of chondrocyte cd44 expression leading to cartilage chondrolysis.
25251539 - Oxidized lipids and lysophosphatidylcholine induce the chemotaxis, up-regulate the expr...
17129169 - Pericellular matrilins regulate activation of chondrocytes by cyclic load-induced matri...
18226989 - Gene expression of jagged2 in mandibular condylar cartilage development.
10382779 - Up-regulated expression of matrilysin and neutrophil collagenase in human herniated discs.
9178349 - Interaction of nicotine and a h2-receptor antagonist, famotidine, on gastrin and chromo...
12606279 - Multiple roles for hedgehog signaling in zebrafish pituitary development.
15289369 - Troponin i isoform expression in human and experimental atrial fibrillation.
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2009-10-28
Journal Detail:
Title:  Journal of cellular and molecular medicine     Volume:  13     ISSN:  1582-4934     ISO Abbreviation:  J. Cell. Mol. Med.     Publication Date:  2009 Sep 
Date Detail:
Created Date:  2010-03-03     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  101083777     Medline TA:  J Cell Mol Med     Country:  England    
Other Details:
Languages:  eng     Pagination:  3398-404     Citation Subset:  IM    
Department of Traumatology, Medical University Vienna, Center for Joint and Cartilage Waehringer Guertel, Vienna, Austria.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Previous Document:  Unusual manifestations of vascular rings.
Next Document:  Isthmin is a novel secreted angiogenesis inhibitor that inhibits tumour growth in mice.