Document Detail

Decreased wild-type full-length Et-A and -B receptors in neuroblastoma and Ewing sarcoma cells.
MedLine Citation:
PMID:  11464869     Owner:  NLM     Status:  MEDLINE    
BACKGROUND: Endothelins and their receptors, Et-A and Et-B, play an essential role in differentiation and migration of neural crest cells. Expression of endothelin receptors has been examined in neuroblastoma and Ewing sarcoma cell lines. PROCEDURE: RNA was amplified for Et-A and Et-B by RT-PCR. Amplified products were cloned into the expression vector pLNCX, which was used to transfect CHO cells. Binding characteristics of transfected CHO cells were examined. RESULTS: Full-length Et-A mRNA was identified in all cell lines, in addition to a truncated Et-A product. CHO cells expressing full-length Et-A bound to endothelin, but cells expressing truncated Et-A did not. Full length Et-B mRNA was not detected, but two smaller molecular weight products were amplified. These are as yet uncharacterised. CONCLUSIONS: These results suggest that endothelins and their receptors may be important in the development and biology of neuroblastoma and Ewing sarcoma.
P A Berry; Y F Zhang; N D Carter; S Jeffery; S A Burchill
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Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Medical and pediatric oncology     Volume:  36     ISSN:  0098-1532     ISO Abbreviation:  Med. Pediatr. Oncol.     Publication Date:  2001 Jan 
Date Detail:
Created Date:  2001-07-23     Completed Date:  2001-08-16     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  7506654     Medline TA:  Med Pediatr Oncol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  142-6     Citation Subset:  IM    
ICRF Cancer Medicine Research Unit, St. James's University Hospital, Leeds, United Kingdom.
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MeSH Terms
Bone Neoplasms / genetics,  metabolism,  pathology*
CHO Cells
Endothelins / metabolism*
Exons / genetics
Gene Expression Regulation, Neoplastic*
Molecular Weight
Neoplasm Proteins / biosynthesis,  chemistry,  genetics*
Neuroblastoma / genetics,  metabolism,  pathology*
Protein Conformation
RNA Splicing
RNA, Messenger / biosynthesis,  genetics
RNA, Neoplasm / biosynthesis,  genetics
Receptor, Endothelin A
Receptor, Endothelin B
Receptors, Endothelin / biosynthesis,  chemistry,  genetics*
Recombinant Fusion Proteins / metabolism
Reverse Transcriptase Polymerase Chain Reaction
Sarcoma, Ewing's / genetics,  metabolism,  pathology*
Sequence Deletion*
Tumor Cells, Cultured / metabolism
Reg. No./Substance:
0/Endothelins; 0/Neoplasm Proteins; 0/RNA, Messenger; 0/RNA, Neoplasm; 0/Receptor, Endothelin A; 0/Receptor, Endothelin B; 0/Receptors, Endothelin; 0/Recombinant Fusion Proteins

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