| Decreased type I collagen expression in human uterine cervix during pregnancy. | |
| | |
MedLine Citation:
|
PMID: 12727979 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
To provide some insight into the mechanism of cervical ripening, the expression of type I collagen was investigated in human uterine cervical tissues obtained from the first (n = 4) and third (n = 3) trimesters of normal pregnancy. Indirect immunofluorescent staining was performed for type I collagen, and Northern blot analysis was done to assess expression of mRNA for the alpha1(I) chain. Collagens were also extracted from the human cervical tissues in the first and third trimesters of pregnancy. Immunohistochemical analysis revealed loose distribution of type I collagen in the uterine cervix of the first trimester compared with the third trimester of pregnancy. The relative levels of various collagens were evaluated by SDS-PAGE. The ratios of the intensity of the band of alpha1(I) to that of total collagen alpha1 chain in cervical tissues of the third trimester were significantly lower than those in cervical tissues of the first trimester of pregnancy (P < 0.05). In contrast, the ratios of the intensity of the band of alpha1(III) to that of total collagen alpha1 chain in cervical tissues of the third trimester were significantly higher than those in cervical tissues of the first trimester of pregnancy (P < 0.05). Northern blot analysis revealed that the cervical expression of mRNA for the alpha1(I) chain was significantly reduced in the third trimester compared with the first trimester of pregnancy (P < 0.01). These results suggest that type I collagen might play an important role in the maintenance of pregnancy and that decreased expression of this collagen could be associated with the process of uterine cervical ripening. |
| | |
Authors:
|
Masaaki Iwahashi; Yasuteru Muragaki; Akira Ooshima; Naohiko Umesaki |
Related Documents
:
|
12070799 - Unrecognized cervical pregnancy treated by suction curettage and cervicovaginal tamponade. 7452159 - Termination of pregnancy by menstrual extraction. 12871889 - Clinical outcomes following interval laparoscopic transabdominal cervico-isthmic cercla... 16625569 - Hyaluronidase for cervical ripening and induction of labour. 8907339 - Development of corticospinal tract fibers and their plasticity i: quantitative analysis... 16256259 - Cervical fetal fibronectin as a predictor of first trimester pregnancy outcome in unexp... 19820049 - Effects of maternal nutrition on conceptus growth and offspring performance: implicatio... 16414699 - Socially available heroin powders--their chemical compositions and reproductive and beh... 12537819 - Does pronuclear morphology and/or early cleavage rate predict embryo implantation poten... |
Publication Detail:
|
Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
|
Title: The Journal of clinical endocrinology and metabolism Volume: 88 ISSN: 0021-972X ISO Abbreviation: J. Clin. Endocrinol. Metab. Publication Date: 2003 May |
Date Detail:
|
Created Date: 2003-05-02 Completed Date: 2003-05-28 Revised Date: 2006-11-15 |
Medline Journal Info:
|
Nlm Unique ID: 0375362 Medline TA: J Clin Endocrinol Metab Country: United States |
Other Details:
|
Languages: eng Pagination: 2231-5 Citation Subset: AIM; IM |
Affiliation:
|
Department of Obstetrics and Gynecology, Wakayama Medical University, Wakayama 641-0012, Japan. masaaki@wakayama-med.ac.jp |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Adult Blotting, Northern Cervix Uteri / chemistry* Collagen Type I / genetics* Electrophoresis, Polyacrylamide Gel Female Fluorescent Antibody Technique, Indirect Gene Expression* Humans Immunohistochemistry Pepsin A / metabolism Pregnancy Pregnancy Trimester, First Pregnancy Trimester, Third RNA, Messenger / analysis |
| Chemical | |
Reg. No./Substance:
|
0/Collagen Type I; 0/RNA, Messenger; EC 3.4.23.1/Pepsin A |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: A genome-wide scan in type 2 diabetes mellitus provides independent replication of a susceptibility ...
Next Document: Endometrial stromal cells undergoing decidualization down-regulate their properties to produce proin...