Document Detail


Decreased ternary complex formation and predominance of a 29 kDa IGFBP-3 fragment in human fetal serum.
MedLine Citation:
PMID:  7524886     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Insulin-like growth factor-I (IGF-I) has been proposed to be important in the endocrine control of fetal growth in humans, although serum IGF-I concentrations are 10-fold lower than during rapid pubertal growth. However, the bioavailability of IGF-I in fetal serum may be increased by changes in the specific IGF binding proteins (IGFBPs). We have recently suggested that the bioavailability of circulating IGF-I is increased in the human fetus due to the molar excess of IGF-I plus IGF-II relative to IGFBP-3 as well as the increased concentrations of IGFBP-2, which does not form a long-lived ternary complex. We have presently studied ternary complex formation between IGF, IGFBP-3, and acid labile subunit (ALS) to further assess if IGF-I bioavailability is increased in human fetal serum. In 19-35 week gestation fetal sera, a markedly decreased formation of the ternary complex was demonstrated by the general absence of IGFBP-3 (detected by Western immunoblotting) in the approximately 130-150 kDa ternary complex after neutral size chromatography. The predominant form of IGFBP-3 in fetal serum was a 29 kDa fragment, which, following deglycosylation by Endoglycosidase-F, was demonstrated to consist of a approximately 20 kDa protein core. Despite the predominance of the 29 kDa IGFBP-3 fragment, we have previously demonstrated that the IGFBP-3 protease activity is not increased in fetal serum, in contrast to pregnancy or non-insulin dependent diabetes mellitus (NIDDM) sera.(ABSTRACT TRUNCATED AT 250 WORDS)
Authors:
P Bang; M Stangenberg; M Westgren; R G Rosenfeld
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Growth regulation     Volume:  4     ISSN:  0956-523X     ISO Abbreviation:  Growth Regul.     Publication Date:  1994 Jun 
Date Detail:
Created Date:  1994-12-01     Completed Date:  1994-12-01     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  9106990     Medline TA:  Growth Regul     Country:  SCOTLAND    
Other Details:
Languages:  eng     Pagination:  68-76     Citation Subset:  IM    
Affiliation:
Department of Endocrinology, Karolinska Institute, Stockholm, Sweden.
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MeSH Terms
Descriptor/Qualifier:
Adult
Biological Availability
Blotting, Western
Carrier Proteins / blood*,  chemistry*,  metabolism
Chromatography / methods
Diabetes Mellitus, Type 2 / blood
Female
Fetal Blood / chemistry*,  metabolism
Growth Inhibitors / blood,  chemistry,  metabolism
Humans
Insulin-Like Growth Factor Binding Proteins
Insulin-Like Growth Factor I / analysis,  pharmacokinetics
Iodine Radioisotopes
Male
Mannosyl-Glycoprotein Endo-beta-N-Acetylglucosaminidase / pharmacology
Molecular Weight
Pregnancy
Grant Support
ID/Acronym/Agency:
HD28703/HD/NICHD NIH HHS
Chemical
Reg. No./Substance:
0/Carrier Proteins; 0/Growth Inhibitors; 0/Insulin-Like Growth Factor Binding Proteins; 0/Iodine Radioisotopes; 67763-96-6/Insulin-Like Growth Factor I; EC 3.2.1.96/Mannosyl-Glycoprotein Endo-beta-N-Acetylglucosaminidase

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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