| Decreased susceptibility to oxidative stress-induced apoptosis of peripheral blood mononuclear cells from healthy elderly and centenarians. | |
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MedLine Citation:
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PMID: 11164477 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The susceptibility to undergo apoptosis of fresh human peripheral blood mononuclear cells (PBMCs) from three groups of healthy donors of different ages: young people (19-40 years), old people (65-85 years) and centenarians was assessed. Apoptosis was induced by 2-deoxy-D-ribose (dRib), an agent which induces apoptosis in quiescent PBMCs by interfering with cell redox status and mitochondrial membrane potential (MMP). Our major finding is that an inverse correlation emerged between the age of the donors and the propensity of their PBMCs to undergo dRib-induced apoptosis. PBMCs from old people and centenarians also showed an increased resistance to dRib-induced glutathione depletion and a decreased tendency to lose MMP. The anti-apoptotic molecule Bcl-2 was similarly expressed in PBMCs from the three age groups. Moreover, the plasma level of the stable product of transglutaminase, epsilon(gamma-glutamyl)lysine isodipeptide, a marker of total body apoptotic rate, was decreased in centenarians compared to young and elderly people. On the whole, these findings suggest that physiological aging is characterised by a decreased tendency to undergo apoptosis, a phenomenon likely resulting from adaptation to lifelong exposure to damaging agents, such as reactive oxygen species, and may contribute to one of the major phenomena of immunosenescence, i.e. the progressive accumulation of memory/effector T cells. |
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Authors:
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D Monti; S Salvioli; M Capri; W Malorni; E Straface; A Cossarizza; B Botti; M Piacentini; G Baggio; C Barbi; S Valensin; M Bonafè; C Franceschi |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Mechanisms of ageing and development Volume: 121 ISSN: 0047-6374 ISO Abbreviation: Mech. Ageing Dev. Publication Date: 2000 Dec |
Date Detail:
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Created Date: 2001-02-22 Completed Date: 2001-05-10 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 0347227 Medline TA: Mech Ageing Dev Country: Ireland |
Other Details:
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Languages: eng Pagination: 239-50 Citation Subset: IM |
Affiliation:
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Department of Experimental Pathology and Oncology, University of Florence, via Morgagni 50, 50139 Florence, Italy. daniela.monti@unifi.it |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Acetylcysteine
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pharmacology Adult Aged Aged, 80 and over Aging / blood* Apoptosis / physiology* Cell Death / drug effects Deoxyribose / pharmacology Dipeptides / blood Drug Resistance Humans Intracellular Membranes / metabolism Membrane Potentials / drug effects Mitochondria / physiology Monocytes / drug effects, metabolism, physiology* Oxidation-Reduction / drug effects Oxidative Stress / physiology* Proto-Oncogene Proteins c-bcl-2 / metabolism Time Factors |
| Chemical | |
Reg. No./Substance:
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0/Dipeptides; 0/Proto-Oncogene Proteins c-bcl-2; 17105-15-6/epsilon-(gamma-glutamyl)-lysine; 533-67-5/Deoxyribose; 616-91-1/Acetylcysteine |
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