| Decreased radioiodine uptake of FRTL-5 cells after (131)I incubation in vitro: molecular biological investigations indicate a cell cycle-dependent pathway. | |
| | |
MedLine Citation:
|
PMID: 18228019 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
PURPOSE: In radioiodine therapy the "stunning phenomenon" is defined as a reduction of radioiodine uptake after diagnostic application of (131)I. In the current study, we established an in vitro model based on the "Fisher rat thyrocyte cell line no. 5" (FRTL-5) to investigate the stunning. METHODS: TSH-stimulated FRTL-5 cells were incubated with (131)I. Time-dependent (131)I uptake and the viability of FRTL-5 cells were evaluated at 4-144 h after radioiodine application. All data was corrected for number of viable cells, half life and (131)I concentration. Sodium iodide symporter (NIS) and the housekeeping gene (beta-actin, GAPDH) levels were quantified by quantitative polymerase chain reaction (qPCR). Additionally, immunohistochemical staining (IHC) of NIS on the cell membrane was carried out. RESULTS: FRTL-5 monolayer cell cultures showed a specific maximum uptake of (131)I 24-48 h after application. Significantly decreased (131)I uptake values were observed after 72-144 h. The decrease in radioiodine uptake was correlated with decreasing mRNA levels of NIS and housekeeping genes. In parallel, unlike in controls, IHC staining of NIS on FRTL-5 cells declined significantly after (131)I long-term incubation. CONCLUSIONS: It could be demonstrated that during (131)I incubation of FRTL-5 cells, radioiodine uptake decreased significantly. Simultaneously decreasing levels of NIS mRNA and protein expression suggest a NIS-associated mechanism. Since mRNA levels of housekeeping genes decreased, too, the reduced NIS expression might be provoked by a cell cycle arrest. Our investigations recommend the FRTL-5 model as a valuable tool for further molecular biological investigations of the stunning phenomenon. |
| | |
Authors:
|
Birgit Meller; Erzsébet Gaspar; Wibke Deisting; Barbara Czarnocka; Manfred Baehre; Björn E Wenzel |
Related Documents
:
|
885559 - Uptake and release of tetracycline by cultured carrot cells. 20720049 - Different modes of transport for 3h-thymidine, 3h-flt, and 3h-fmau in proliferating and... 14530519 - Pendrin transporter carries out iodide uptake into mcf-7 human mammary cancer cells. 22316479 - Immunophenotyping of classic murine myeloma cell lines used for monoclonal antibody pro... 2162849 - Sodium-dependent phosphate transport in primary cultures of renal tubule cells from you... 16665849 - Aluminum effects on uptake and metabolism of phosphorus by the cyanobacterium anabaena ... 8138979 - Stereological and immunohistochemical study of development of human fetal labial saliva... 20541599 - Cellular responses by stable and uniform ultrafine titanium dioxide particles in cultur... 23358229 - Non-apoptotic toxicity of pseudomonas aeruginosa toward murine cells. |
Publication Detail:
|
Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2008-01-29 |
Journal Detail:
|
Title: European journal of nuclear medicine and molecular imaging Volume: 35 ISSN: 1619-7070 ISO Abbreviation: Eur. J. Nucl. Med. Mol. Imaging Publication Date: 2008 Jun |
Date Detail:
|
Created Date: 2008-05-14 Completed Date: 2008-10-14 Revised Date: - |
Medline Journal Info:
|
Nlm Unique ID: 101140988 Medline TA: Eur J Nucl Med Mol Imaging Country: Germany |
Other Details:
|
Languages: eng Pagination: 1204-12 Citation Subset: IM |
Affiliation:
|
Clinic of Radiology and Nuclear Medicine, University of Lübeck, Ratzeburger Allee 160, 23538, Lübeck, Germany. Birgit.Meller@uk-sh.de |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Animals Cell Cycle / radiation effects* Cell Line Iodine Radioisotopes / administration & dosage*, pharmacokinetics* Rats Rats, Inbred F344 Signal Transduction / radiation effects* Thyroid Gland / metabolism*, radiation effects* |
| Chemical | |
Reg. No./Substance:
|
0/Iodine Radioisotopes |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: An imaging analysis of (99m)Tc-UBI (29-41) uptake in S. aureus infected thighs of rabbits on ciprofl...
Next Document: CD34+ leukemic subpopulation predominantly displays lower spontaneous apoptosis and has higher expre...