Document Detail


Decreased production of interferon gamma by anti-CD3 monoclonal antibody and interleukin-2-stimulated peripheral blood mononuclear cells in Hodgkin's disease.
MedLine Citation:
PMID:  8555074     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
We have examined the production of interferon (IFN) gamma by peripheral blood mononuclear cells (PBMC) in 22 patients with Hodgkin's disease (HD) in active phase of the disease, 12 patients in clinical remission and 16 healthy subjects. The level of IFN gamma in supernatants of PBMC stimulated for 72 h with anti-CD3 monoclonal antibody (mAb), measured by sandwich enzyme immunoassay (ELISA) was 50.4 +/- 2.3 U/ml in active phase; 137.0 +/- 7.4 U/ml in clinical remission patients; and 520.0 +/- 10.0 U/ml in the controls; the difference between the groups was statistically significant. Co-stimulation with interleukin-2 (rIL-2) markedly amplified production of IFN gamma. The mean levels were 220.8 +/- 7.0 U/ml, 590.7 +/- 3.6 U/ml and 2111.1 +/- 17.3 U/ml in active phase HD, clinical remission and controls, respectively, the difference between groups was statistically significant. The patients showed the same kinetic pattern as healthy individuals. Our results indicate that patients with HD have severely impaired TCR/CD3 activation pathway resulting in significantly depressed IFN gamma response to anti-CD3 mAb and anti-CD3 + rIL-2 in vitro stimulation and provide support for the possible clinical use of IFN gamma as an immunopotentiating agent in patients with HD.
Authors:
I Frydecka; G Mazur; K Kuliczkowski
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  British journal of haematology     Volume:  91     ISSN:  0007-1048     ISO Abbreviation:  Br. J. Haematol.     Publication Date:  1995 Nov 
Date Detail:
Created Date:  1996-02-23     Completed Date:  1996-02-23     Revised Date:  2008-11-21    
Medline Journal Info:
Nlm Unique ID:  0372544     Medline TA:  Br J Haematol     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  671-3     Citation Subset:  IM    
Affiliation:
Department of Haematology, Medical University, Wrocław, Poland.
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MeSH Terms
Descriptor/Qualifier:
Adult
Aged
Enzyme-Linked Immunosorbent Assay
Female
Hodgkin Disease / metabolism*
Humans
Interferon-gamma / metabolism*
Interleukin-2 / pharmacology*
Leukocytes, Mononuclear / metabolism*
Male
Middle Aged
Muromonab-CD3 / pharmacology*
Chemical
Reg. No./Substance:
0/Interleukin-2; 0/Muromonab-CD3; 82115-62-6/Interferon-gamma

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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