| Decreased phosphorylation of STAT-1, STAT-4 and cytokine release in MDR-TB patients with primary resistance. | |
| | |
MedLine Citation:
|
PMID: 18713507 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
SETTING: We recently showed that treatment failure rate was higher among multidrug-resistant tuberculosis (MDR-TB) patients without a previous history of tuberculosis (TB) treatment, or so-called 'primary resistance'. OBJECTIVE: To investigate the phosphorylation levels of signal transducers and activators of transcription-1 (STAT-1) and STAT-4 and the subsequent cytokine release as a possible cause of a poor prognosis in MDR-TB patients with primary resistance. DESIGN: Ten patients with successfully treated pulmonary TB without resistance, 12 MDR-TB patients with acquired resistance and 10 MDR-TB patients with primary resistance were enrolled. After 24 h stimulation of peripheral blood mononuclear cells (PBMC) with interferon-gamma (IFN-gamma), interleukin-12 (IL-12), purified protein derivative (PPD), or lysate of Mycobacterium tuberculosis, flow cytometric analysis of intracellular pSTAT-1 and pSTAT-4 were performed and secretion of IFN-gamma, IL-12p40 and tumour necrosis factor-alpha (TNF-alpha) was measured in culture supernatant. RESULTS: The mean fluorescent intensities of pSTAT-1 and pSTAT-4 in PBMC of MDR-TB patients with primary resistance decreased on stimulation of IFN-gamma, PPD or lysate of M. tuberculosis when compared with patients with acquired resistance. In addition, secretion of IFN-gamma, IL-12p40 and TNF-alpha in these patients decreased on various stimuli. CONCLUSION: Decreased phosphorylation of STAT-1, STAT-4, and of subsequent cytokine release, might be associated with a poor prognosis in MDR-TB patients with primary resistance. |
| | |
Authors:
|
H J Kim; D H Chung; M J Kim; J H Jang; Y W Kim; S K Han; Y-S Shim; J-J Yim |
Related Documents
:
|
16396697 - The efficiency of switching from infliximab to etanercept and vice-versa in patients wi... 11602907 - Differences in arterial and mixed venous il-6 levels: the lungs as a source of cytokine... 3418087 - The clinical significance of serum c reactive protein levels in crohn's disease. result... 16151917 - The ratio of alpha-galactosidase to beta-glucuronidase activities in dried blood for th... 18155597 - Burning mouth syndrome and burning mouth in hypothyroidism: proposal for a diagnostic a... 7624407 - Use of the orbicularis muscle flap for complex lower lid problems: a 6-year analysis. |
Publication Detail:
|
Type: Comparative Study; Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
|
Title: The international journal of tuberculosis and lung disease : the official journal of the International Union against Tuberculosis and Lung Disease Volume: 12 ISSN: 1027-3719 ISO Abbreviation: Int. J. Tuberc. Lung Dis. Publication Date: 2008 Sep |
Date Detail:
|
Created Date: 2008-08-20 Completed Date: 2008-12-16 Revised Date: - |
Medline Journal Info:
|
Nlm Unique ID: 9706389 Medline TA: Int J Tuberc Lung Dis Country: France |
Other Details:
|
Languages: eng Pagination: 1071-6 Citation Subset: IM |
Affiliation:
|
Division of Pulmonary and Critical Care Medicine, Department of Internal Medicine and Lung Institute, Seoul National University College of Medicine, Seoul, Republic of Korea. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Adolescent Adult Cytokines / metabolism* Enzyme-Linked Immunosorbent Assay Female Flow Cytometry Humans Leukocytes, Mononuclear / chemistry, immunology Male Middle Aged Mycobacterium tuberculosis / immunology* Phosphorylation STAT1 Transcription Factor / metabolism* STAT4 Transcription Factor / metabolism* Tuberculosis, Multidrug-Resistant / blood, drug therapy, immunology, metabolism* Tuberculosis, Pulmonary / drug therapy, immunology*, metabolism, microbiology |
| Chemical | |
Reg. No./Substance:
|
0/Cytokines; 0/STAT1 Transcription Factor; 0/STAT1 protein, human; 0/STAT4 Transcription Factor; 0/STAT4 protein, human |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Risk factors for multidrug-resistant tuberculosis in Hong Kong.
Next Document: Knowledge, attitudes and practice of university students regarding waterpipe smoking in Pakistan.