| Decreased phospholipase A2 activity and prostaglandin biosynthesis in Bacillus Calmette-Guérin-activated alveolar macrophages. | |
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MedLine Citation:
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PMID: 7041985 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Alveolar macrophages from normal and BCG-infected rabbits were labelled with [14C]arachidonate and its metabolites were measured. It was found that production of both prostaglandins and 12-hydroxyeicosatetraenoic acid was diminished markedly in BCG-primed macrophages. This decrease was due partly to a depression in cyclooxygenase and lipoxygenase activity, but mainly to a decrease in arachidonic acid release, probably due to a suppression in phospholipase A2 activity. This is indicated by a consistent depression of this enzyme activity in BCG macrophage homogenates at a wide pH range, suggesting that both lysosomal an non-lysosomal phospholipases are suppressed in BCG macrophages. However, intracellular lysosomal acid phosphatase and its release were increased markedly in BCG-primed macrophages. Our previous studies have shown a close relationship between lysosomal acid hydrolase release and production of arachidonate-prostaglandins in normal macrophages. The present study shows that in activated macrophages primed by BCG, different mechanisms are operative in the control of synthesis and release of lysosomal acid hydrolases and of the phospholipase for prostaglandin production. |
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Authors:
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W Hsueh; R Lamb; F Gonzalez-Crussi |
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Publication Detail:
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Type: Journal Article; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
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Title: Biochimica et biophysica acta Volume: 710 ISSN: 0006-3002 ISO Abbreviation: Biochim. Biophys. Acta Publication Date: 1982 Mar |
Date Detail:
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Created Date: 1982-07-08 Completed Date: 1982-07-08 Revised Date: 2007-11-15 |
Medline Journal Info:
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Nlm Unique ID: 0217513 Medline TA: Biochim Biophys Acta Country: NETHERLANDS |
Other Details:
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Languages: eng Pagination: 406-14 Citation Subset: IM |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Arachidonic Acids / metabolism Kinetics Macrophages / metabolism*, microbiology Male Mycobacterium bovis* Phagocytosis Phospholipases / metabolism* Phospholipases A / metabolism* Phospholipases A2 Phospholipids / biosynthesis Prostaglandins / biosynthesis* Rabbits Triglycerides / biosynthesis |
| Grant Support | |
ID/Acronym/Agency:
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AI 17419/AI/NIAID NIH HHS; R23 AI 16147/AI/NIAID NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Arachidonic Acids; 0/Phospholipids; 0/Prostaglandins; 0/Triglycerides; EC 3.1.-/Phospholipases; EC 3.1.1.-/Phospholipases A; EC 3.1.1.4/Phospholipases A2 |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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