Document Detail

Decreased orexigenic response to neuropeptide Y in rats with obstructive cholestasis.
MedLine Citation:
PMID:  11171627     Owner:  NLM     Status:  MEDLINE    
Neuropeptide Y (NPY) is a key factor in the neurochemical control of food intake, and obstructive cholestasis can be associated with disturbances in food intake. Our aim in this study was to determine whether obstructive cholestasis in the rat is associated with defective central responsiveness to NPY. Cholestasis was induced in rats by surgical bile duct resection. Rats with obstructive cholestasis exhibited a 20% reduction in food intake 2 days after laparotomy (compared with sham-resected controls) that had resolved by 4 days after surgery. Responsiveness to the orexigenic action of NPY was tested by measuring food intake after intracerebroventricular injection of NPY. In sham-resected rats, NPY infusion strikingly increased food intake, whereas bile duct-resected (BDR) rats showed a consistent significantly impaired feeding response to NPY at postlaparotomy days 2, 4, and 7. Separate experiments measured specific binding of [(3)H]NPY to hypothalamic receptors. Fos protein expression was measured in the hypothalamic paraventricular nucleus (PVN) as a marker of NPY-induced neuronal activation. The decreased orexigenic responsiveness to NPY was not caused by altered NPY binding at hypothalamic receptors or its ability to activate neurons in the PVN. Therefore, cholestatic rats demonstrate an attenuated NPY-induced orexigenic drive that occurs early after biliary obstruction, when cholestatic rats exhibit reduced food intake, and persists despite the return of food intake to normal levels and the presence of intact central NPY-related neuronal pathways.
K P Rioux; T Le; M G Swain
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  American journal of physiology. Gastrointestinal and liver physiology     Volume:  280     ISSN:  0193-1857     ISO Abbreviation:  Am. J. Physiol. Gastrointest. Liver Physiol.     Publication Date:  2001 Mar 
Date Detail:
Created Date:  2001-02-22     Completed Date:  2001-03-22     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  100901227     Medline TA:  Am J Physiol Gastrointest Liver Physiol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  G449-56     Citation Subset:  IM    
Liver Unit, Gastrointestinal Research Group, University of Calgary, Health Sciences Centre, Calgary, Alberta, Canada T2N 4N1.
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MeSH Terms
Bile Ducts / physiology,  surgery
Binding, Competitive / drug effects
Cholestasis / drug therapy*,  metabolism
Disease Models, Animal
Dose-Response Relationship, Drug
Drinking / drug effects
Eating / drug effects*
Gastric Emptying / drug effects
Hypothalamus / metabolism
Injections, Intraventricular
Neuropeptide Y / administration & dosage*,  metabolism
Proto-Oncogene Proteins c-fos / biosynthesis
Rats, Sprague-Dawley
Receptors, Neuropeptide Y / metabolism
Reg. No./Substance:
0/Neuropeptide Y; 0/Proto-Oncogene Proteins c-fos; 0/Receptors, Neuropeptide Y

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