Document Detail


Decreased muscarinic M1 receptor gene expression in the hypothalamus, brainstem, and pancreatic islets of streptozotocin-induced diabetic rats.
MedLine Citation:
PMID:  17960828     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The brain neurotransmitters' receptor activity and hormonal pathways control many physiological functions in the body. Acetylcholine (ACh), a major neurotransmitter from autonomic nervous system, regulates the cholinergic stimulation of insulin secretion, through interactions with muscarinic receptors. The objective of the present study was to investigate the changes in the total muscarinic and muscarinic M1 receptor ([(3)H]quinuclidinyl benzilate; QNB) binding and gene expression in the hypothalamus, brainstem, and pancreatic islets of streptozotocin (STZ)-induced diabetic and insulin-treated diabetic rats. In the hypothalamus and brainstem, total muscarinic receptor numbers were increased in diabetic rats with increase in affinity. Hypothalamic and brainstem muscarinic M1 receptors number were decreased in STZ diabetic rats with increase in affinity. In the pancreatic islets, muscarinic M1 receptors of diabetic rats were decreased, with a decrease in affinity. In all cases, the binding parameters were reversed to near control by the treatment of diabetic rats with insulin. Real-time PCR data also showed a decrease in muscarinic M1 receptor gene expression and a similar reversal with insulin treatment. Thus our results suggest that insulin modulates binding parameters and gene expression of total and muscarinic M1 receptors.
Authors:
G Gireesh; S Balarama Kaimal; T Peeyush Kumar; C S Paulose
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Journal of neuroscience research     Volume:  86     ISSN:  1097-4547     ISO Abbreviation:  J. Neurosci. Res.     Publication Date:  2008 Mar 
Date Detail:
Created Date:  2008-02-18     Completed Date:  2008-07-08     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  7600111     Medline TA:  J Neurosci Res     Country:  United States    
Other Details:
Languages:  eng     Pagination:  947-53     Citation Subset:  IM    
Copyright Information:
(c) 2007 Wiley-Liss, Inc.
Affiliation:
Molecular Neurobiology and Cell Biology Unit, Centre for Neuroscience, Cochin University of Science and Technology, Cochin, Kerala, India.
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MeSH Terms
Descriptor/Qualifier:
Animals
Blood Glucose / drug effects
Brain Stem / drug effects,  metabolism*
Diabetes Mellitus, Experimental / drug therapy,  metabolism*
Gene Expression / drug effects
Hypothalamus / drug effects,  metabolism*
Insulin / therapeutic use
Islets of Langerhans / drug effects,  metabolism*
Male
Rats
Rats, Wistar
Receptor, Muscarinic M1 / biosynthesis*,  drug effects,  genetics
Reverse Transcriptase Polymerase Chain Reaction
Chemical
Reg. No./Substance:
0/Blood Glucose; 0/Receptor, Muscarinic M1; 11061-68-0/Insulin

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