| Decreased mucosal expression of intestinal alkaline phosphatase in children with coeliac disease. | |
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MedLine Citation:
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PMID: 22262031 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
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A major function of the enzyme intestinal alkaline phosphatase (iAP) is the detoxification of lipopolysaccharide (LPS), the ligand of Toll-like receptor 4 (TLR4). Hence, iAP has a role in the defence of maintaining intestinal barrier integrity. As intestinal barrier integrity is impaired in coeliac disease (CD), we tested the expression and localization of iAP in duodenal mucosa specimens from children with newly diagnosed CD (n = 10), with CD on gluten-free diet (GFD) (n = 5) and compared to those from ten healthy children. The mRNA and protein expression was determined by RT-PCR and Western blot analysis, respectively. Tissue localization of iAP and TLR4 was determined by immunofluorescence staining. iAP protein expression level was significantly lower than normal in newly diagnosed CD, while it was normalised in children on GFD. iAP and TLR4 colocalized at the epithelial surface of duodenal mucosa in each group of subjects enrolled. The finding of decreased iAP protein levels in newly diagnosed CD is consistent with its role in decreased intestinal barrier integrity. The latter may be the result of decreased LPS-detoxifying ability. |
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Authors:
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Kriszta Molnár; Adám Vannay; Erna Sziksz; Nóra Fanni Bánki; Hajnalka Győrffy; András Arató; Antal Dezsőfi; Gabor Veres |
Publication Detail:
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Type: JOURNAL ARTICLE Date: 2012-1-20 |
Journal Detail:
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Title: Virchows Archiv : an international journal of pathology Volume: - ISSN: 1432-2307 ISO Abbreviation: - Publication Date: 2012 Jan |
Date Detail:
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Created Date: 2012-1-20 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 9423843 Medline TA: Virchows Arch Country: - |
Other Details:
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Languages: ENG Pagination: - Citation Subset: - |
Affiliation:
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1st Department of Paediatrics, Semmelweis University, Bókay u. 53, H-1083, Budapest, Hungary. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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