Document Detail


Decreased metastatic phenotype in cells resistant to aminolevulinic acid-photodynamic therapy.
MedLine Citation:
PMID:  18662847     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Photodynamic therapy (PDT) is a novel cancer treatment utilising a photosensitiser, visible light and oxygen. PDT often leaves a significant number of surviving tumour cells. In a previous work, we isolated and studied two PDT resistant clones derived from the mammary adenocarcinoma LM3 line (Int. J. Oncol. 29 (2006) 397-405). The isolated Clon 4 and Clon 8 exhibited a more fibroblastic, dendritic pattern and were larger than the parentals. In the present work we studied the metastatic potential of the two clones in comparison with LM3. We found that 100% of LM3 invaded Matrigel, whereas only 19+/-6% and 24+/-7% of Clon 4 and Clon 8 cells invaded. In addition, 100% of LM3 cells migrated towards a chemotactic stimulus whereas 38+/-8% and 73+/-10% of Clones 4 and 8, respectively, were able to migrate. In vivo, 100% of the LM3 injected mice developed spontaneous lung metastasis, whereas none of the Clon 8 did, and only one of the mice injected with Clon 4 did. No differences were found in the proteolytic enzyme profiles among the cells. Anchorage-dependent adhesion was also impaired in vivo in the resistant clones, evidenced by the lower tumour take, latency time and growth rates, although both clones showed in vitro higher binding to collagen I without overexpression of beta1 integrin. This is the first work where the metastatic potential of cells surviving to PDT has been studied. PDT strongly affects the invasive phenotype of these cells, probably related to a higher binding to collagen. These findings may be crucial for the outcome of ALA-PDT of metastatic tumours, although further studies are needed to extrapolate the results to the clinic employing another photosensitisers and cell types.
Authors:
Adriana Casas; Gabriela Di Venosa; Silvia Vanzulli; Christian Perotti; Leandro Mamome; Lorena Rodriguez; Marina Simian; Angeles Juarranz; Osvaldo Pontiggia; Tayyaba Hasan; Alcira Batlle
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S.     Date:  2008-07-26
Journal Detail:
Title:  Cancer letters     Volume:  271     ISSN:  1872-7980     ISO Abbreviation:  Cancer Lett.     Publication Date:  2008 Nov 
Date Detail:
Created Date:  2008-10-13     Completed Date:  2008-10-28     Revised Date:  2013-06-05    
Medline Journal Info:
Nlm Unique ID:  7600053     Medline TA:  Cancer Lett     Country:  Ireland    
Other Details:
Languages:  eng     Pagination:  342-51     Citation Subset:  IM    
Affiliation:
Centro de Investigaciones sobre Porfirinas y Porfirias, CONICET and Hospital de Clínicas José de San Martín, University of Buenos Aires, 1056 Ciudad de Buenos Aires, Córdoba 2351 1er subsuelo, CP 1120AAF, Buenos Aires, Argentina. adriana@qb.fcen.uba.ar
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MeSH Terms
Descriptor/Qualifier:
Aminolevulinic Acid / therapeutic use*
Animals
Cell Adhesion
Drug Resistance, Neoplasm
Lung Neoplasms / pathology,  secondary
Male
Mice
Mice, Inbred BALB C
Neoplasm Metastasis*
Photochemotherapy*
Grant Support
ID/Acronym/Agency:
P01 CA084203/CA/NCI NIH HHS; P01 CA084203-05/CA/NCI NIH HHS; P01 CA84203/CA/NCI NIH HHS; R01 AR040352/AR/NIAMS NIH HHS
Chemical
Reg. No./Substance:
106-60-5/Aminolevulinic Acid
Comments/Corrections

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