Document Detail

Decreased hydrogen-potassium-activated ATPase (H+-K+-ATPase) expression and gastric acid secretory capacity in aged mice.
MedLine Citation:
PMID:  17240463     Owner:  NLM     Status:  MEDLINE    
Gastric acid secretion in response to chemical stimulation and to mechanical stimulation was investigated in adult and old mice. The protein expression of a proton pump (H(+)-K(+)-ATPase), a marker of parietal cell function, was determined by Western blotting. Acid secretion was stimulated by histamine (500 and 1000 microg/kg) or carbachol (10 and 20 microg/kg). To investigate the response to mechanical stimulation, the stomach was distended by an intragastric injection of isotonic saline (0.5, 1.0, 1.5, and 2.0 ml). Administration of two doses of histamine produced a dose-dependent increase in acid secretion in adult mice, whereas a higher dose of histamine failed to produce a further increase in old mice. Gastric acid secretion, whether produced by carbachol or mechanical stimulation, did not differ between the two age groups. The protein expression of H(+)-K(+)-ATPase was significantly lower in old mice than in adult. Insofar as histamine increases acid secretion via the cyclic AMP (cAMP) pathway in parietal cells, while carbachol and gastric distention do so via the calcium signaling pathway, the cAMP signaling pathway may be more susceptible to aging than the calcium signaling pathway. The decrease in the secretory capacity of acid secretion in the old mice may be partly attributable to a decrease in parietal cell function, as shown by decrease in H(+)-K(+)-ATPase protein expression.
Setsuko Kanai; Hiroko Hosoya; Minoru Ohta; Kyoko Miyasaka
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2007-01-19
Journal Detail:
Title:  Archives of gerontology and geriatrics     Volume:  45     ISSN:  0167-4943     ISO Abbreviation:  Arch Gerontol Geriatr     Publication Date:    2007 Nov-Dec
Date Detail:
Created Date:  2007-09-25     Completed Date:  2007-11-27     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8214379     Medline TA:  Arch Gerontol Geriatr     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  243-52     Citation Subset:  IM    
Department of Clinical Physiology, Tokyo Metropolitan Institute of Gerontology, 35-2 Sakaecho Itabashiku, Tokyo l73-0015, Japan.
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MeSH Terms
Adenosine Triphosphatases / metabolism*
Aging / physiology*
Blotting, Western
Carbachol / pharmacology
Gastric Acid / secretion
Histamine / pharmacology
Mice, Inbred C57BL
Parietal Cells, Gastric / physiology
Reg. No./Substance:
51-45-6/Histamine; 51-83-2/Carbachol; EC 3.6.1.-/Adenosine Triphosphatases

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