Document Detail


Decreased expression of microRNA-199b increases protein levels of SET (protein phosphatase 2A inhibitor) in human choriocarcinoma.
MedLine Citation:
PMID:  19900756     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
We compared microRNA profiles between choriocarcinoma and non-cancerous trophoblasts, and revealed that miR-199b was underexpressed in choriocarcinoma. By computational prediction and microarray studies, SET (protein phosphatase 2A inhibitor) was shown to be one of the target genes regulated by miR-199b. Ectopic expression of miR-199b inhibited endogenous SET protein levels and the activity of the luciferase reporter containing the 3'-UTR of SET. Further comparisons of formalin-fixed paraffin-embedded human choriocarcinoma, mole, and non-cancer trophoblast tissues confirmed the initial findings of low miR-199b expression and SET upregulation in choriocarcinomas, suggesting that microRNA-dysregulated SET protein may account for the rapid growth seen with choriocarcinomas.
Authors:
Angel Chao; Chia-Lung Tsai; Pei-Chi Wei; Swei Hsueh; An-Shine Chao; Chin-Jung Wang; Chi-Neu Tsai; Yun-Shien Lee; Tzu-Hao Wang; Chyong-Huey Lai
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2009-11-08
Journal Detail:
Title:  Cancer letters     Volume:  291     ISSN:  1872-7980     ISO Abbreviation:  Cancer Lett.     Publication Date:  2010 May 
Date Detail:
Created Date:  2010-03-29     Completed Date:  2010-04-16     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  7600053     Medline TA:  Cancer Lett     Country:  Ireland    
Other Details:
Languages:  eng     Pagination:  99-107     Citation Subset:  IM    
Copyright Information:
2009 Elsevier Ireland Ltd. All rights reserved.
Affiliation:
Department of Obstetrics and Gynecology, Chang Gung Memorial Hospital and Chang Gung University, Taiwan.
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MeSH Terms
Descriptor/Qualifier:
Cell Line, Tumor
Cell Proliferation
Choriocarcinoma / chemistry,  genetics*,  pathology
Female
Histone Chaperones / analysis,  genetics,  physiology*
Humans
MicroRNAs / analysis,  physiology*
Pregnancy
Transcription Factors / analysis,  genetics,  physiology*
Trophoblasts / chemistry
Uterine Neoplasms / chemistry,  genetics*,  pathology
Chemical
Reg. No./Substance:
0/Histone Chaperones; 0/MicroRNAs; 0/SET protein, human; 0/Transcription Factors; 0/mirn199 microRNA, human

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