Document Detail


Decreased expression of C-erbB-2 and CXCR4 in breast cancer after primary chemotherapy.
MedLine Citation:
PMID:  23046610     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
BACKGROUND: Biological molecular markers such as proto-oncogene erbB-2 (HER-2/neu, c-erbB-2), the CXC chemokine receptor 4 (CXCR4), estrogen receptor (ER), Proliferating Cell Nuclear Antigen (PCNA), DNA topoisomerase II (topo II), P-glycoprotein (P-gp) and glutathione S-transferase (GST) were observed for changes after administration of neochemotherapy and whether these protein expression changes were correlated with response to chemotherapy.
METHODS: Sixty-four patients with primary breast cancer who had undergone neo-adjuvant chemotherapy were enrolled in the present study. The expressions of C-erbB-2, CXCR4 and ER-α were measured by immunohistochemistry (IHC) on full tissue sections and on tissue microarrays (TMAs). PCNA, TopoII, P-gp and GST were measured by IHC on TMAs. On the other hand, CXCR4, C-erbB-2 and ER-α expressions were detected using western blot analysis to 16 pairs of fresh preoperative core biopsies. The final surgical specimens were obtained from patients with breast carcinoma who received neo-adjuvant chemotherapy and obtained a partial response (PR).
RESULTS: Our data demonstrated that the levels of C-erbB-2, CXCR4 and ER-α in patients decreased after they received neo-adjuvant chemotherapy on full tissue sections and on TMAs. The PCNA level was down-regulated after receiving neo-adjuvant chemotherapy, and no significant change was observed for TopoII, P-gp and GST. The levels of C-erbB-2, CXCR4 and ER-α were also down-regulated after neo-adjuvant chemotherapy was administered, as detected by western blot. In addition, the change expressions of C-erbB-2 and CXCR4 in specimens tended to be correlated with pathological change to neo-adjuvant chemotherapy on full tissue sections and on TMAs in a Pearson chi-square analysis.
CONCLUSIONS: As demonstrated in our study, after breast cancer patients were treated with neo-adjuvant systemic therapy, decreased expressions of C-erbB2, ER-α and CXCR4 were observed. Down-regulated expressions of c-erbB-2 and CXCR4 may be a novel mechanism of chemotherapy; the changes of these objective markers may be useful in evaluating the clinical response of neo-adjuvant chemotherapy in breast cancer.
Authors:
Shi-Xin Yang; Wings T Y Loo; Louis W C Chow; Xin-hua Yang; Yi Zhan; Lin-Jun Fan; Fan Zhang; Li Chen; Qing-liang Wang; Hua-Liang Xiao; Jin-Long Wu; Xiu-wu Bian; Jun Jiang
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2012-09-19
Journal Detail:
Title:  Journal of translational medicine     Volume:  10 Suppl 1     ISSN:  1479-5876     ISO Abbreviation:  J Transl Med     Publication Date:  2012 Sep 
Date Detail:
Created Date:  2012-10-10     Completed Date:  2013-05-14     Revised Date:  2013-07-11    
Medline Journal Info:
Nlm Unique ID:  101190741     Medline TA:  J Transl Med     Country:  England    
Other Details:
Languages:  eng     Pagination:  S3     Citation Subset:  IM    
Affiliation:
Breast Disease Center, Southwest Hospital, Third Military Medical University, Chongqing, PR China.
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MeSH Terms
Descriptor/Qualifier:
Adult
Aged
Blotting, Western
Breast Neoplasms / drug therapy*,  pathology
Estrogen Receptor alpha / metabolism
Female
Humans
Immunohistochemistry
Middle Aged
Receptor, erbB-2 / metabolism*
Receptors, CXCR4 / metabolism*
Staining and Labeling
Treatment Outcome
Young Adult
Chemical
Reg. No./Substance:
0/CXCR4 protein, human; 0/Estrogen Receptor alpha; 0/Receptors, CXCR4; 0/estrogen receptor alpha, human; EC 2.7.10.1/ERBB2 protein, human; EC 2.7.10.1/Receptor, erbB-2
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