| Decreased efficiency of trypsinization of cells following photodynamic therapy: evaluation of a role for tissue transglutaminase. | |
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MedLine Citation:
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PMID: 11202365 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Identifying the cellular responses to photodynamic therapy (PDT) is important if the mechanisms of cellular damage are to be fully understood. The relationship between sensitizer, fluence rate and the removal of cells by trypsinization was studied using the RIF-1 cell line. Following treatment of RIF-1 cells with pyridinium zinc (II) phthalocyanine (PPC), or polyhaematoporphyrin at 10 mW cm-2 (3 J cm-2), there was a significant number of cells that were not removed by trypsin incubation compared to controls. Decreasing the fluence rate from 10 to 2.5 mW cm-2 resulted in a two-fold increase in the number of cells attached to the substratum when PPC used as sensitizer; however, with 5,10,15,20 meso-tetra(hydroxyphenyl) chlorine (m-THPC) there was no resistance to trypsinization following treatment at either fluence rate. The results indicate that resistance of cells to trypsinization following PDT is likely to be both sensitizer and fluence rate dependent. Increased activity of the enzyme tissue-transglutaminase (tTGase) was observed following PPC-PDT, but not following m-THPC-PDT. Similar results were obtained using HT29 human colonic carcinoma and ECV304 human umbilical vein endothelial cell lines. Hamster fibrosarcoma cell (Met B) clones transfected with human tTGase also exhibited resistance to trypsinization following PPC-mediated photosensitization; however, a similar degree of resistance was observed in PDT-treated control Met B cells suggesting that tTGase activity alone was not involved in this process. |
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Authors:
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D J Ball; S Mayhew; D I Vernon; M Griffin; S B Brown |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Photochemistry and photobiology Volume: 73 ISSN: 0031-8655 ISO Abbreviation: Photochem. Photobiol. Publication Date: 2001 Jan |
Date Detail:
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Created Date: 2001-02-01 Completed Date: 2001-03-08 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 0376425 Medline TA: Photochem Photobiol Country: United States |
Other Details:
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Languages: eng Pagination: 47-53 Citation Subset: IM |
Affiliation:
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Center for Photobiology and Photodynamic Therapy, School of Biochemistry and Molecular Biology, University of Leeds, Leeds LS2 9JT, UK. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Cell Adhesion Cell Line Cricetinae Humans Mice Photochemotherapy* Transfection Transglutaminases / genetics, metabolism Tretinoin / pharmacology Trypsin |
| Chemical | |
Reg. No./Substance:
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302-79-4/Tretinoin; EC 2.3.2.13/Transglutaminases; EC 3.4.21.4/Trypsin |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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