Document Detail

Decreased efficiency of gamma-ray-induced DNA double-strand break rejoining in malignant transformants of human bronchial epithelial cells generated by alpha-particle exposure.
MedLine Citation:
PMID:  12428918     Owner:  NLM     Status:  MEDLINE    
PURPOSE: To investigate the cytogenetic changes and DNA double-strand break (DSB) rejoining of transformed cell lines generated from human bronchial epithelial cells by alpha-particle exposure. MATERIALS AND METHODS: Transformed cell lines were derived from the HPV 18-immortalized human bronchial epithelial cell line BEP2D generated by 1.5 Gy of alpha-particles emitted by a 238Pu source. Two cell lines, BERP35T1 and BERP35T4, were investigated. Karyotypes were analyzed by trypsin/Giemsa banding. Cell survival was estimated by colony assay. PFGE was used to detect the DNA DSB. mRNA expression was analyzed by RT-PCR. RESULTS: Abnormal chromosomes 2 and 12 with elongated long arm and deletions of chromosomes 2, 12, 13 and 17 were observed in the transformed cell lines. BERP35T4 showed a much higher proportion of polyploid cells (40.5%) compared with parental BEP2D cells and the BERP35TI cell line (5%). BERP35T1 and BERP35T4 showed a markedly lower capacity for rejoining of gamma-ray-induced DNA DSB and increased radiosensitivity compared with parental BEP2D cells. The analysis of mRNA levels revealed a 2.5- to 6.5-fold down-regulated expression of the DNA repair genes XRCC-2, XRCC-3 and Ku80 in BERP35T1 and BERP35T4 cells. CONCLUSION: The karyotypic changes of chromosomes 2, 12, 13 and 17 and the deficiency of DSB rejoining could be related to the malignant transformation processing of BEP2D cells initiated by alpha-particle exposure.
J F Sun; J L Sui; P K Zhou; Y Geng; Y C Hu; Z S Cao; S L Ge; T Z Lou; D C Wu
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  International journal of radiation biology     Volume:  78     ISSN:  0955-3002     ISO Abbreviation:  Int. J. Radiat. Biol.     Publication Date:  2002 Sep 
Date Detail:
Created Date:  2002-11-13     Completed Date:  2002-12-02     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  8809243     Medline TA:  Int J Radiat Biol     Country:  England    
Other Details:
Languages:  eng     Pagination:  773-80     Citation Subset:  IM; S    
Department of Radiation Toxicology, Beijing Institute of Radiation Medicine, Beijing, PR China.
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MeSH Terms
Alpha Particles / adverse effects
Bronchi / metabolism,  radiation effects
Cell Line, Transformed
Cell Survival / radiation effects
Cell Transformation, Neoplastic
DNA / genetics,  radiation effects*
DNA Damage*
DNA Repair / genetics
Epithelial Cells / metabolism,  radiation effects
Gamma Rays / adverse effects
Gene Expression / radiation effects
Papillomaviridae / pathogenicity
RNA, Messenger / genetics,  metabolism
Reg. No./Substance:
0/RNA, Messenger; 9007-49-2/DNA

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