| Decreased cytochrome oxidase activity in hepatic mitochondria after chronic ethanol consumption and the possible role of decreased cytochrome aa3 content and changes in phospholipids. | |
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MedLine Citation:
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PMID: 6320904 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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In ethanol-fed baboons, hepatic mitochondrial cytochrome oxidase activity and cytochrome aa3 content were significantly decreased by 58.3 and 50.5%, respectively, compared to their pair-fed controls. However, there was no significant correlation between the two, suggesting that other factors in addition to cytochrome aa3 may be responsible for the depression in cytochrome oxidase activity. The total phospholipid content of the mitochondrial membranes was significantly decreased (0.24 +/- 0.03 mumol of phospholipid phosphorus/mg of protein vs. 0.32 +/- 0.04 in controls). This change was accounted for, in part, by the significant decrease in the levels of phosphatidylcholine and cardiolipin. In addition, the fatty acid pattern of the phospholipids was changed. There was a marked increase in the relative amounts of oleic and linoleic acids and a decrease in arachidonic acid. These changes were associated with an increase in the activity of phospholipase A2. The reactivation rate of phospholipid-depleted cytochrome oxidase by endogenous phospholipids from ethanol-fed baboons was significantly lower than that by phospholipid from pair-fed controls, when measured at an optimal phospholipid to protein ratio. Thus, it appears that alterations in the phospholipid composition of the mitochondrial membranes are responsible, at least in part, for the depression of cytochrome oxidase activity produced by chronic ethanol consumption. |
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Authors:
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M Arai; E R Gordon; C S Lieber |
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Publication Detail:
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Type: Journal Article; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
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Title: Biochimica et biophysica acta Volume: 797 ISSN: 0006-3002 ISO Abbreviation: Biochim. Biophys. Acta Publication Date: 1984 Mar |
Date Detail:
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Created Date: 1984-04-12 Completed Date: 1984-04-12 Revised Date: 2007-11-15 |
Medline Journal Info:
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Nlm Unique ID: 0217513 Medline TA: Biochim Biophys Acta Country: NETHERLANDS |
Other Details:
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Languages: eng Pagination: 320-7 Citation Subset: IM |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Electron Transport Complex IV / metabolism* Ethanol / pharmacology* Fatty Acids / analysis Mitochondria, Liver / drug effects, enzymology* Papio Phospholipases A / metabolism Phospholipases A2 Phospholipids / metabolism* |
| Grant Support | |
ID/Acronym/Agency:
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AA-03508/AA/NIAAA NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Fatty Acids; 0/Phospholipids; 64-17-5/Ethanol; EC 1.9.3.1/Electron Transport Complex IV; EC 3.1.1.-/Phospholipases A; EC 3.1.1.4/Phospholipases A2 |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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