Document Detail


Decreased citrate improves iron availability from infant formula: application of an in vitro digestion/Caco-2 cell culture model.
MedLine Citation:
PMID:  9446853     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
We have applied an in vitro digestion/Caco-2 cell culture model to the assessment of iron availability from human milk and a generic cow's milk-based infant formula. Experiments were designed to determine the availability of iron from human milk relative to infant formula and whether known promoters of iron absorption would increase Caco-2 cell iron uptake and availability from the infant formula. In addition, we sought to determine if decreasing the citrate concentration in the infant formula would increase the iron uptake. Although approximately twice as much iron was in solution from digests of the infant formula relative to that of human milk, smaller or equal amounts of iron were taken up from the infant formula relative to the human milk digest. These results are qualitatively similar to in vivo studies. Addition of known iron uptake promoters to infant formula did not enhance Caco-2 cell iron uptake from the infant formula digest, indicating that the iron in the infant formula existed predominantly in a tightly bound unavailable form(s). Enzymatic pretreatment of the infant formula with citrate lyase and oxalacetate decarboxylase decreased the citrate concentration by 67% and resulted in a 64% increase of iron in solution, which corresponded to a 46% increase in the cell iron uptake. Iron uptake from the "low citrate" formula plus cysteine was 102% greater relative to the nontreated formula. The results indicate that too much citrate can reduce iron uptake, particularly if it is present at concentrations greater than promoters such as ascorbic acid and cysteine.
Authors:
R P Glahn; C Lai; J Hsu; J F Thompson; M Guo; D R Van Campen
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Publication Detail:
Type:  Comparative Study; Journal Article    
Journal Detail:
Title:  The Journal of nutrition     Volume:  128     ISSN:  0022-3166     ISO Abbreviation:  J. Nutr.     Publication Date:  1998 Feb 
Date Detail:
Created Date:  1998-03-05     Completed Date:  1998-03-05     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0404243     Medline TA:  J Nutr     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  257-64     Citation Subset:  IM    
Affiliation:
U.S. Plant, Soil and Nutrition Laboratory, USDA/ARS, Tower Road, Ithaca, NY 14853, USA.
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MeSH Terms
Descriptor/Qualifier:
Animals
Biological Availability
Caco-2 Cells / metabolism*
Cattle
Cells, Cultured
Citrates / administration & dosage,  analysis,  pharmacology*
Digestion / drug effects
Humans
Infant Food* / analysis
Iron / analysis,  pharmacokinetics*
Milk, Human / chemistry,  metabolism*
Chemical
Reg. No./Substance:
0/Citrates; 7439-89-6/Iron

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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