Document Detail


Decreased blood pressure and vascular smooth muscle tone in mice lacking basolateral Na(+)-K(+)-2Cl(-) cotransporter.
MedLine Citation:
PMID:  12384462     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The basolateral Na(+)-K(+)-2Cl(-) cotransporter (NKCC1) functions in the maintenance of cellular electrolyte and volume homeostasis. NKCC1-deficient (Nkcc1(-/-)) mice were used to examine its role in cardiac function and in the maintenance of blood pressure and vascular tone. Tail-cuff measurements demonstrated that awake Nkcc1(-/-) mice had significantly lower systolic blood pressure than wild-type (Nkcc1(+/+)) mice (114.5 +/- 2.2 and 131.8 +/- 2.5 mmHg, respectively). Serum aldosterone levels were normal, indicating that extracellular fluid-volume homeostasis was not impaired. Studies using pressure transducers in the femoral artery and left ventricle showed that anesthetized Nkcc1(-/-) mice have decreased mean arterial pressure and left ventricular pressure, whereas myocardial contraction parameters were not significantly different from those of Nkcc1(+/+) mice. When stimulated with phenylephrine, aortic smooth muscle from Nkcc1(+/+) and Nkcc1(-/-) mice exhibited no significant differences in maximum contractility and only moderate dose-response shifts. In phasic portal vein smooth muscle from Nkcc1(-/-) mice, however, a sharp reduction in mechanical force was noted. These results indicate that NKCC1 can be important for the maintenance of normal blood pressure and vascular tone.
Authors:
Jamie W Meyer; Michael Flagella; Roy L Sutliff; John N Lorenz; Michelle L Nieman; Craig S Weber; Richard J Paul; Gary E Shull
Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  American journal of physiology. Heart and circulatory physiology     Volume:  283     ISSN:  0363-6135     ISO Abbreviation:  Am. J. Physiol. Heart Circ. Physiol.     Publication Date:  2002 Nov 
Date Detail:
Created Date:  2002-10-17     Completed Date:  2002-11-22     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  100901228     Medline TA:  Am J Physiol Heart Circ Physiol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  H1846-55     Citation Subset:  IM    
Affiliation:
Department of Molecular Genetics, Biochemistry, and Microbiology, University of Cincinnati College of Medicine, 231 Albert Sabin Way, Cincinnati, OH 45267, USA.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:
Acid-Base Equilibrium / physiology
Aldosterone / blood
Animals
Blood Pressure / physiology*
Bumetanide / pharmacology
Diuretics / pharmacology
Electrolytes / blood
Hypotension / metabolism,  physiopathology*
Mice
Mice, Knockout
Muscle Tonus / drug effects,  physiology
Muscle, Smooth, Vascular / physiology*
Phenotype
Sodium-Potassium-Chloride Symporters / genetics*,  metabolism*
Stress, Mechanical
Tail
Grant Support
ID/Acronym/Agency:
DK 50594/DK/NIDDK NIH HHS; DK 57552/DK/NIDDK NIH HHS; HL 61974/HL/NHLBI NIH HHS
Chemical
Reg. No./Substance:
0/Diuretics; 0/Electrolytes; 0/Sodium-Potassium-Chloride Symporters; 0/sodium-potassium-chloride cotransporter 1 protein; 28395-03-1/Bumetanide; 52-39-1/Aldosterone

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Abnormal cardiac function associated with sympathetic nervous system hyperactivity in mice.
Next Document:  Postexercise hypotension in conscious SHR is attenuated by blockade of substance P receptors in NTS.