| Decreased blood pressure and vascular smooth muscle tone in mice lacking basolateral Na(+)-K(+)-2Cl(-) cotransporter. | |
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MedLine Citation:
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PMID: 12384462 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The basolateral Na(+)-K(+)-2Cl(-) cotransporter (NKCC1) functions in the maintenance of cellular electrolyte and volume homeostasis. NKCC1-deficient (Nkcc1(-/-)) mice were used to examine its role in cardiac function and in the maintenance of blood pressure and vascular tone. Tail-cuff measurements demonstrated that awake Nkcc1(-/-) mice had significantly lower systolic blood pressure than wild-type (Nkcc1(+/+)) mice (114.5 +/- 2.2 and 131.8 +/- 2.5 mmHg, respectively). Serum aldosterone levels were normal, indicating that extracellular fluid-volume homeostasis was not impaired. Studies using pressure transducers in the femoral artery and left ventricle showed that anesthetized Nkcc1(-/-) mice have decreased mean arterial pressure and left ventricular pressure, whereas myocardial contraction parameters were not significantly different from those of Nkcc1(+/+) mice. When stimulated with phenylephrine, aortic smooth muscle from Nkcc1(+/+) and Nkcc1(-/-) mice exhibited no significant differences in maximum contractility and only moderate dose-response shifts. In phasic portal vein smooth muscle from Nkcc1(-/-) mice, however, a sharp reduction in mechanical force was noted. These results indicate that NKCC1 can be important for the maintenance of normal blood pressure and vascular tone. |
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Authors:
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Jamie W Meyer; Michael Flagella; Roy L Sutliff; John N Lorenz; Michelle L Nieman; Craig S Weber; Richard J Paul; Gary E Shull |
Publication Detail:
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Type: Journal Article; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
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Title: American journal of physiology. Heart and circulatory physiology Volume: 283 ISSN: 0363-6135 ISO Abbreviation: Am. J. Physiol. Heart Circ. Physiol. Publication Date: 2002 Nov |
Date Detail:
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Created Date: 2002-10-17 Completed Date: 2002-11-22 Revised Date: 2007-11-14 |
Medline Journal Info:
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Nlm Unique ID: 100901228 Medline TA: Am J Physiol Heart Circ Physiol Country: United States |
Other Details:
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Languages: eng Pagination: H1846-55 Citation Subset: IM |
Affiliation:
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Department of Molecular Genetics, Biochemistry, and Microbiology, University of Cincinnati College of Medicine, 231 Albert Sabin Way, Cincinnati, OH 45267, USA. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Acid-Base Equilibrium
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physiology Aldosterone / blood Animals Blood Pressure / physiology* Bumetanide / pharmacology Diuretics / pharmacology Electrolytes / blood Hypotension / metabolism, physiopathology* Mice Mice, Knockout Muscle Tonus / drug effects, physiology Muscle, Smooth, Vascular / physiology* Phenotype Sodium-Potassium-Chloride Symporters / genetics*, metabolism* Stress, Mechanical Tail |
| Grant Support | |
ID/Acronym/Agency:
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DK 50594/DK/NIDDK NIH HHS; DK 57552/DK/NIDDK NIH HHS; HL 61974/HL/NHLBI NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Diuretics; 0/Electrolytes; 0/Sodium-Potassium-Chloride Symporters; 0/sodium-potassium-chloride cotransporter 1 protein; 28395-03-1/Bumetanide; 52-39-1/Aldosterone |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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