| Decreased accumulation of glutelin types in rice grains constitutively expressing a sunflower seed albumin gene. | |
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MedLine Citation:
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PMID: 16242165 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Previous studies have shown differential accumulation of sulfur rich glutelins and sulfur poor prolamins in transgenic rice seeds expressing a sunflower seed albumin gene [Hagan, N.D., Upadhyaya, N., Tabe, L.M., Higgins, T.J., 2003. The redistribution of protein sulfur in transgenic rice expressing a gene for a foreign, sulfur-rich protein. Plant J 34, 1-11]. Here, we show, by two-dimensional electrophoresis, differential accumulation of three classes of glutelin proteins - type I, II and III - and a globulin, not previously resolved, in transgenic seeds grown under low and high sulfur nutrition. Several glutelin polypeptides were resolved and four identified as a type I glutelin, two type II glutelins and a type III glutelin. Although sulfur nutrition did not affect the accumulation of sunflower seed albumin, the levels of all four identified glutelins and the globulin were lower in mature seeds derived from transgenic plants grown under sulfur-optimum or sulfur limited conditions compared to non-transgenic rice seeds. The reduction of all four glutelin polypeptides and the globulin varied from 21% to 68%. The re-allocation of sulfur reserves from endogenous proteins to the sulfur sink in transgenic grain is suggestive of a transcriptional control of sulfur mobilization in plants. |
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Authors:
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Nazrul Islam; Narayana M Upadhyaya; Peter M Campbell; Ray Akhurst; Nick Hagan; Thomas J V Higgins |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2005-10-19 |
Journal Detail:
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Title: Phytochemistry Volume: 66 ISSN: 0031-9422 ISO Abbreviation: Phytochemistry Publication Date: 2005 Nov |
Date Detail:
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Created Date: 2005-11-01 Completed Date: 2006-07-25 Revised Date: 2008-11-21 |
Medline Journal Info:
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Nlm Unique ID: 0151434 Medline TA: Phytochemistry Country: United States |
Other Details:
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Languages: eng Pagination: 2534-9 Citation Subset: IM |
Affiliation:
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CSIRO Entomology, Clunies Ross Street, GPO Box 1700, Canberra, ACT 2601, Australia. naz.islam@csiro.au |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Albumins
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genetics*,
metabolism Gene Expression Regulation, Plant / genetics Glutens / metabolism* Helianthus / genetics* Oryza sativa / genetics*, metabolism* Plants, Genetically Modified Seeds / genetics* Sulfur |
| Chemical | |
Reg. No./Substance:
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0/Albumins; 7704-34-9/Sulfur; 8002-80-0/Glutens |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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