Document Detail


Decreased RB1 mRNA, protein, and activity reflect obesity-induced altered adipogenic capacity in human adipose tissue.
MedLine Citation:
PMID:  23315497     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Retinoblastoma (Rb1) has been described as an essential player in white adipocyte differentiation in mice. No studies have been reported thus far in human adipose tissue or human adipocytes. We aimed to investigate the possible role and regulation of RB1 in adipose tissue in obesity using human samples and animal and cell models. Adipose RB1 (mRNA, protein, and activity) was negatively associated with BMI and insulin resistance (HOMA-IR) while positively associated with the expression of adipogenic genes (PPARγ and IRS1) in both visceral and subcutaneous human adipose tissue. BMI increase was the main contributor to adipose RB1 downregulation. In rats, adipose Rb1 gene expression and activity decreased in parallel to dietary-induced weight gain and returned to baseline with weight loss. RB1 gene and protein expression and activity increased significantly during human adipocyte differentiation. In fully differentiated adipocytes, transient knockdown of Rb1 led to loss of the adipogenic phenotype. In conclusion, Rb1 seems to play a permissive role for human adipose tissue function, being downregulated in obesity and increased during differentiation of human adipocytes. Rb1 knockdown findings further implicate Rb1 as necessary for maintenance of adipogenic characteristics in fully differentiated adipocytes.
Authors:
José María Moreno-Navarrete; Petar Petrov; Marta Serrano; Francisco Ortega; Estefanía García-Ruiz; Paula Oliver; Joan Ribot; Wifredo Ricart; Andreu Palou; M Luisa Bonet; José Manuel Fernández-Real
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2013-01-11
Journal Detail:
Title:  Diabetes     Volume:  62     ISSN:  1939-327X     ISO Abbreviation:  Diabetes     Publication Date:  2013 Jun 
Date Detail:
Created Date:  2013-05-24     Completed Date:  2013-08-02     Revised Date:  2013-12-23    
Medline Journal Info:
Nlm Unique ID:  0372763     Medline TA:  Diabetes     Country:  United States    
Other Details:
Languages:  eng     Pagination:  1923-31     Citation Subset:  AIM; IM    
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MeSH Terms
Descriptor/Qualifier:
Adipogenesis / genetics,  physiology
Adipose Tissue / metabolism*
Adiposity / genetics,  physiology
Adult
Female
Humans
Immunoblotting
Male
Middle Aged
Obesity / genetics,  metabolism*
RNA, Messenger / genetics*
Retinoblastoma / genetics,  metabolism*
Chemical
Reg. No./Substance:
0/RNA, Messenger

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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