| Decreased HIV diversity after allogeneic stem cell transplantation of an HIV-1 infected patient: a case report. | |
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MedLine Citation:
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PMID: 20210988 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The human immunodeficiency virus type 1 (HIV-1) coreceptor use and viral evolution were analyzed in blood samples from an HIV-1 infected patient undergoing allogeneic stem cell transplantation (SCT). Coreceptor use was predicted in silico from sequence data obtained from the third variable loop region of the viral envelope gene with two software tools. Viral diversity and evolution was evaluated on the same samples by Bayesian inference and maximum likelihood methods. In addition, phenotypic analysis was done by comparison of viral growth in peripheral blood mononuclear cells and in a CCR5 (R5)-deficient T-cell line which was controlled by a reporter assay confirming viral tropism. In silico coreceptor predictions did not match experimental determinations that showed a consistent R5 tropism. Anti-HIV directed antibodies could be detected before and after the SCT. These preexisting antibodies did not prevent viral rebound after the interruption of antiretroviral therapy during the SCT. Eventually, transplantation and readministration of anti-retroviral drugs lead to sustained increase in CD4 counts and decreased viral load to undetectable levels. Unexpectedly, viral diversity decreased after successful SCT. Our data evidence that only R5-tropic virus was found in the patient before and after transplantation. Therefore, blocking CCR5 receptor during stem cell transplantation might have had beneficial effects and this might apply to more patients undergoing allogeneic stem cell transplantation. Furthermore, we revealed a scenario of HIV-1 dynamic different from the commonly described ones. Analysis of viral evolution shows the decrease of viral diversity even during episodes with bursts in viral load. |
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Authors:
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Christel Kamp; Timo Wolf; Ignacio G Bravo; Benjamin Kraus; Birgit Krause; Britta Neumann; Gudrun Winskowsky; Alexander Thielen; Albrecht Werner; Barbara S Schnierle |
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Publication Detail:
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Type: Case Reports; Journal Article; Research Support, Non-U.S. Gov't Date: 2010-03-08 |
Journal Detail:
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Title: Virology journal Volume: 7 ISSN: 1743-422X ISO Abbreviation: Virol. J. Publication Date: 2010 |
Date Detail:
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Created Date: 2010-03-26 Completed Date: 2010-04-21 Revised Date: 2010-09-30 |
Medline Journal Info:
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Nlm Unique ID: 101231645 Medline TA: Virol J Country: England |
Other Details:
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Languages: eng Pagination: 55 Citation Subset: IM |
Affiliation:
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Paul-Ehrlich-Institut, Paul-Ehrlich-Strasse 51-59, 63225 Langen, Germany. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Adult Anti-HIV Agents / therapeutic use CD4 Lymphocyte Count Cell Line Cells, Cultured Cluster Analysis Genetic Variation* HIV Antibodies / blood HIV Envelope Protein gp120 / genetics HIV Infections / drug therapy, virology* HIV-1 / classification*, genetics, growth & development, isolation & purification* Humans Male Phylogeny Receptors, HIV / analysis Sequence Analysis, DNA Sequence Homology Stem Cell Transplantation* T-Lymphocytes / virology |
| Chemical | |
Reg. No./Substance:
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0/Anti-HIV Agents; 0/HIV Antibodies; 0/HIV Envelope Protein gp120; 0/Receptors, HIV; 0/gp120 protein, Human immunodeficiency virus 1 |
| Comments/Corrections | |
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