Document Detail


Decreased GABA receptor binding in the cerebral cortex of insulin induced hypoglycemic and streptozotocin induced diabetic rats.
MedLine Citation:
PMID:  20532620     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Hypoglycemia is the major problem to blood glucose homeostasis in treatment of diabetes and is associated with severe irreversible consequences including seizures, coma and death. GABAergic inhibitory function in the cerebral cortex plays an important role in controlling the excitability and responsiveness of cortical neurons. Present study analysed effects of insulin induced hypoglycemia and streptozotocin induced diabetes on the cortical GABA receptor binding, GABA(Aά1), GABA(B) receptor subtype expression, GAD and GLUT3 expression. Diabetic rats showed decreased [(3)H] GABA binding in the cerebral cortex compared to control while hypoglycemia exacerbated the decrease. GABA receptor subunits; GABA(Aά1), GABA(B) and GAD expression significantly decreased in diabetic rats whereas hypoglycemia significanly decreased the expression compared to diabetic. GLUT3 expression significantly up regulated during both hypo and hyperglycemia. Our results showed that hypoglycemia and hyperglycemia decreased GABAergic neuroprotective function in the cerebral cortex, which account for the increased vulnerability of cerebral cortex to subsequent neuronal damage during hypo/hyperglycemia.
Authors:
Sherin Antony; T Peeyush Kumar; Korah P Kuruvilla; Naijil George; C S Paulose
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2010-06-08
Journal Detail:
Title:  Neurochemical research     Volume:  35     ISSN:  1573-6903     ISO Abbreviation:  Neurochem. Res.     Publication Date:  2010 Oct 
Date Detail:
Created Date:  2010-09-20     Completed Date:  2011-01-18     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  7613461     Medline TA:  Neurochem Res     Country:  United States    
Other Details:
Languages:  eng     Pagination:  1516-21     Citation Subset:  IM    
Affiliation:
Centre for Neuroscience, Department of Biotechnology, Cochin University of Science and Technology, Cochin, Kerala, India.
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MeSH Terms
Descriptor/Qualifier:
Animals
Blood Glucose / metabolism
Cerebral Cortex / metabolism*
Diabetes Mellitus, Experimental / chemically induced,  metabolism*
Gene Expression Regulation
Glutamate Decarboxylase / biosynthesis,  genetics
Hypoglycemia / chemically induced,  metabolism*
Insulin*
Male
Protein Binding
Protein Subunits / biosynthesis,  genetics
Rats
Rats, Wistar
Receptors, GABA / biosynthesis*,  genetics
Streptozocin*
Chemical
Reg. No./Substance:
0/Blood Glucose; 0/Protein Subunits; 0/Receptors, GABA; 11061-68-0/Insulin; 18883-66-4/Streptozocin; EC 4.1.1.15/Glutamate Decarboxylase

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