| Decreased Cytochrome c Oxidase Subunit VIIa in Aged Rat Heart Mitochondria: Immunocytochemistry. | |
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MedLine Citation:
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PMID: 21972221 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
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Aging decreases oxidative phosphorylation through cytochrome oxidase (COX) in cardiac interfibrillar mitochondria (IFM) in 24-month old (aged) rats compared to 6-month old adult Fischer 344 rats, whereas subsarcolemmal mitochondria (SSM) located beneath the plasma membrane remain unaffected. Immunoelectron microscopy (IEM) reveals in aged rats a 25% reduction in cardiac COX subunit VIIa in cardiac IFM, but not in SSM. In contrast, the content of subunit IV remains unchanged in both SSM and IFM, irrespective of age. These subunits are localized mainly on cristae membranes. In contrast, semi-quantitative immunoblotting, which detects denatured protein, indicates that the content of COX VIIa is similar in IFM and SSM from both aged and adult hearts. IEM provides a sensitive method for precise localizing and quantifying specific mitochondrial proteins. The lack of immunoreaction of COX VIIa subunit by IEM in aged IFM is not explained by a reduction in protein, but rather by a masking phenomenon or by an in situ change in protein structure affecting COX activity. Anat Rec, 2011. © 2011 Wiley-Liss, Inc. |
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Authors:
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Hisashi Fujioka; Shadi Moghaddas; Deborah G Murdock; Edward J Lesnefsky; Bernard Tandler; Charles L Hoppel |
Publication Detail:
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Type: JOURNAL ARTICLE Date: 2011-10-4 |
Journal Detail:
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Title: Anatomical record (Hoboken, N.J. : 2007) Volume: - ISSN: 1932-8494 ISO Abbreviation: - Publication Date: 2011 Oct |
Date Detail:
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Created Date: 2011-10-5 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 101292775 Medline TA: Anat Rec (Hoboken) Country: - |
Other Details:
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Languages: ENG Pagination: - Citation Subset: - |
Copyright Information:
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Copyright © 2011 Wiley-Liss, Inc. |
Affiliation:
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Department of Pharmacology, School of Medicine, Case Western Reserve University, Cleveland, Ohio; Electron Microscope Facility, School of Medicine, Case Western Reserve University, Cleveland, Ohio. hisashi.fujioka@case.edu. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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