Document Detail

Decreased cytochrome c oxidase subunit VIIa in aged rat heart mitochondria: immunocytochemistry.
MedLine Citation:
PMID:  21972221     Owner:  NLM     Status:  MEDLINE    
Aging decreases oxidative phosphorylation through cytochrome oxidase (COX) in cardiac interfibrillar mitochondria (IFM) in 24-month old (aged) rats compared to 6-month old adult Fischer 344 rats, whereas subsarcolemmal mitochondria (SSM) located beneath the plasma membrane remain unaffected. Immunoelectron microscopy (IEM) reveals in aged rats a 25% reduction in cardiac COX subunit VIIa in cardiac IFM, but not in SSM. In contrast, the content of subunit IV remains unchanged in both SSM and IFM, irrespective of age. These subunits are localized mainly on cristae membranes. In contrast, semi-quantitative immunoblotting, which detects denatured protein, indicates that the content of COX VIIa is similar in IFM and SSM from both aged and adult hearts. IEM provides a sensitive method for precise localizing and quantifying specific mitochondrial proteins. The lack of immunoreaction of COX VIIa subunit by IEM in aged IFM is not explained by a reduction in protein, but rather by a masking phenomenon or by an in situ change in protein structure affecting COX activity.
Hisashi Fujioka; Shadi Moghaddas; Deborah G Murdock; Edward J Lesnefsky; Bernard Tandler; Charles L Hoppel
Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural     Date:  2011-10-04
Journal Detail:
Title:  Anatomical record (Hoboken, N.J. : 2007)     Volume:  294     ISSN:  1932-8494     ISO Abbreviation:  Anat Rec (Hoboken)     Publication Date:  2011 Nov 
Date Detail:
Created Date:  2011-10-14     Completed Date:  2012-02-01     Revised Date:  2013-08-20    
Medline Journal Info:
Nlm Unique ID:  101292775     Medline TA:  Anat Rec (Hoboken)     Country:  United States    
Other Details:
Languages:  eng     Pagination:  1825-33     Citation Subset:  IM    
Copyright Information:
Copyright © 2011 Wiley-Liss, Inc.
Department of Pharmacology, School of Medicine, Case Western Reserve University, Cleveland, Ohio 44106, USA.
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MeSH Terms
Age Factors
Aging / metabolism*
Blotting, Western
Electron Transport Complex IV / metabolism*
Microscopy, Immunoelectron*
Mitochondria, Heart / enzymology*,  ultrastructure
Oxidative Phosphorylation
Rats, Inbred F344
Grant Support
P01 AG015885/AG/NIA NIH HHS; P01 AG015885/AG/NIA NIH HHS; P01 AG015885-09/AG/NIA NIH HHS
Reg. No./Substance:
EC Transport Complex IV

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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