| Decrease of peritoneal inflammatory CD4(+), CD8(+), CD19(+) lymphocytes and apoptosis of eosinophils in a murine Taenia crassiceps infection. | |
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MedLine Citation:
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PMID: 20706737 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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After an intraperitoneal infection of mice with Taenia crassiceps metacestodes, peritoneal inflammatory cells labeled with fluoresceinated MoAb anti-mouse were analyzed by flow cytometry. Apoptosis was studied by annexin A/PI, TUNEL assays, DNA laddering, caspase-3 activity, and electron microscopy. An important continuous decrease of CD4+, CD8+ and CD19+ lymphocytes, and an increase of eosinophils and macrophages throughout the observation time were found. Apoptosis of eosinophils was quantified during the observation period with a peak at 6 days post-infection (67.27%). In an additional experiment at 12 days post-infection using TUNEL staining, a high level of apoptosis of eosinophil (92.3%) and a significant decrease of CD4+, CD8+, and CD19+ lymphocytes were confirmed. Caspase-3 activity in peritoneal fluid, peritoneal cells' DNA fragmentation, and apoptosis of eosinophils and monocytes were found. The dramatic decrease of peritoneal inflammatory T and B cells and the high level of apoptosis of inflammatory eosinophils induced in mice by infection with T. crassiceps cysticerci may be important factors of the immunosuppression observed in cysticercosis. |
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Authors:
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Nadia Zepeda; Sandra Solano; Natalia Copitin; Ana María Fernández; Lilián Hernández; Patricia Tato; José L Molinari |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2010-08-13 |
Journal Detail:
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Title: Parasitology research Volume: 107 ISSN: 1432-1955 ISO Abbreviation: Parasitol. Res. Publication Date: 2010 Oct |
Date Detail:
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Created Date: 2010-10-04 Completed Date: 2011-01-06 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 8703571 Medline TA: Parasitol Res Country: Germany |
Other Details:
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Languages: eng Pagination: 1129-35 Citation Subset: IM |
Affiliation:
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Departamento de Bioquímica y Biología Estructural, Instituto de Fisiología Celular, Universidad Nacional Autónoma de México, México, DF 04510, Ap. Postal 70-242, Mexico. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Antigens, CD19 / analysis Apoptosis* CD8-Positive T-Lymphocytes / immunology Disease Models, Animal Eosinophils / immunology* Female Flow Cytometry Mice Mice, Inbred BALB C Microscopy, Electron Peritonitis / immunology*, parasitology, pathology* T-Lymphocyte Subsets / immunology* Taenia / isolation & purification Taeniasis / immunology*, parasitology, pathology* Time Factors |
| Chemical | |
Reg. No./Substance:
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0/Antigens, CD19 |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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