Document Detail

Decrease of peritoneal inflammatory CD4(+), CD8(+), CD19(+) lymphocytes and apoptosis of eosinophils in a murine Taenia crassiceps infection.
MedLine Citation:
PMID:  20706737     Owner:  NLM     Status:  MEDLINE    
After an intraperitoneal infection of mice with Taenia crassiceps metacestodes, peritoneal inflammatory cells labeled with fluoresceinated MoAb anti-mouse were analyzed by flow cytometry. Apoptosis was studied by annexin A/PI, TUNEL assays, DNA laddering, caspase-3 activity, and electron microscopy. An important continuous decrease of CD4+, CD8+ and CD19+ lymphocytes, and an increase of eosinophils and macrophages throughout the observation time were found. Apoptosis of eosinophils was quantified during the observation period with a peak at 6 days post-infection (67.27%). In an additional experiment at 12 days post-infection using TUNEL staining, a high level of apoptosis of eosinophil (92.3%) and a significant decrease of CD4+, CD8+, and CD19+ lymphocytes were confirmed. Caspase-3 activity in peritoneal fluid, peritoneal cells' DNA fragmentation, and apoptosis of eosinophils and monocytes were found. The dramatic decrease of peritoneal inflammatory T and B cells and the high level of apoptosis of inflammatory eosinophils induced in mice by infection with T. crassiceps cysticerci may be important factors of the immunosuppression observed in cysticercosis.
Nadia Zepeda; Sandra Solano; Natalia Copitin; Ana María Fernández; Lilián Hernández; Patricia Tato; José L Molinari
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2010-08-13
Journal Detail:
Title:  Parasitology research     Volume:  107     ISSN:  1432-1955     ISO Abbreviation:  Parasitol. Res.     Publication Date:  2010 Oct 
Date Detail:
Created Date:  2010-10-04     Completed Date:  2011-01-06     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8703571     Medline TA:  Parasitol Res     Country:  Germany    
Other Details:
Languages:  eng     Pagination:  1129-35     Citation Subset:  IM    
Departamento de Bioquímica y Biología Estructural, Instituto de Fisiología Celular, Universidad Nacional Autónoma de México, México, DF 04510, Ap. Postal 70-242, Mexico.
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MeSH Terms
Antigens, CD19 / analysis
CD8-Positive T-Lymphocytes / immunology
Disease Models, Animal
Eosinophils / immunology*
Flow Cytometry
Mice, Inbred BALB C
Microscopy, Electron
Peritonitis / immunology*,  parasitology,  pathology*
T-Lymphocyte Subsets / immunology*
Taenia / isolation & purification
Taeniasis / immunology*,  parasitology,  pathology*
Time Factors
Reg. No./Substance:
0/Antigens, CD19

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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