Document Detail


Decrease of peritoneal inflammatory CD4(+), CD8(+), CD19(+) lymphocytes and apoptosis of eosinophils in a murine Taenia crassiceps infection.
MedLine Citation:
PMID:  20706737     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
After an intraperitoneal infection of mice with Taenia crassiceps metacestodes, peritoneal inflammatory cells labeled with fluoresceinated MoAb anti-mouse were analyzed by flow cytometry. Apoptosis was studied by annexin A/PI, TUNEL assays, DNA laddering, caspase-3 activity, and electron microscopy. An important continuous decrease of CD4+, CD8+ and CD19+ lymphocytes, and an increase of eosinophils and macrophages throughout the observation time were found. Apoptosis of eosinophils was quantified during the observation period with a peak at 6 days post-infection (67.27%). In an additional experiment at 12 days post-infection using TUNEL staining, a high level of apoptosis of eosinophil (92.3%) and a significant decrease of CD4+, CD8+, and CD19+ lymphocytes were confirmed. Caspase-3 activity in peritoneal fluid, peritoneal cells' DNA fragmentation, and apoptosis of eosinophils and monocytes were found. The dramatic decrease of peritoneal inflammatory T and B cells and the high level of apoptosis of inflammatory eosinophils induced in mice by infection with T. crassiceps cysticerci may be important factors of the immunosuppression observed in cysticercosis.
Authors:
Nadia Zepeda; Sandra Solano; Natalia Copitin; Ana María Fernández; Lilián Hernández; Patricia Tato; José L Molinari
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2010-08-13
Journal Detail:
Title:  Parasitology research     Volume:  107     ISSN:  1432-1955     ISO Abbreviation:  Parasitol. Res.     Publication Date:  2010 Oct 
Date Detail:
Created Date:  2010-10-04     Completed Date:  2011-01-06     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8703571     Medline TA:  Parasitol Res     Country:  Germany    
Other Details:
Languages:  eng     Pagination:  1129-35     Citation Subset:  IM    
Affiliation:
Departamento de Bioquímica y Biología Estructural, Instituto de Fisiología Celular, Universidad Nacional Autónoma de México, México, DF 04510, Ap. Postal 70-242, Mexico.
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MeSH Terms
Descriptor/Qualifier:
Animals
Antigens, CD19 / analysis
Apoptosis*
CD8-Positive T-Lymphocytes / immunology
Disease Models, Animal
Eosinophils / immunology*
Female
Flow Cytometry
Mice
Mice, Inbred BALB C
Microscopy, Electron
Peritonitis / immunology*,  parasitology,  pathology*
T-Lymphocyte Subsets / immunology*
Taenia / isolation & purification
Taeniasis / immunology*,  parasitology,  pathology*
Time Factors
Chemical
Reg. No./Substance:
0/Antigens, CD19

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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