| Decrease in rat internal anal pressure with the use of a topical ointment containing a killed E. coli culture suspension. | |
| | |
MedLine Citation:
|
PMID: 16628409 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
OBJECTIVES: The present study aimed to clarify the mechanisms of a topical ointment containing an Escherichia coli culture suspension and hydrocortisone (Posterisan forte, BCS+HC) in lowering internal anal pressure in conscious rats. MATERIALS AND METHODS: Internal anal pressure was measured using a water-filled balloon system for consecutive 10-min periods. The changes in pressure were evaluated by the number of peaks above 20 mmH2O between 1 and 8 min of recording. RESULTS: Topical intra-anal application of BCS+HC ointment (160 mg/kg) significantly decreased the internal anal pressure at 3 h after the application. Thereafter, this effect reached a maximum decrease at 4 h and lasted until 6 h. BCS+HC ointment (40, 80, and 160 mg/kg) lowered the internal anal pressure at 4-5 h in a dose-dependent manner. The maximum decrease ratios of the ointment and corresponding hydrocortisone-free ointment (Posterisan, BCS) were 32.6+/-12.7 and 25.7+/-9.0%, respectively, revealing significant pressure-lowering effects compared with a placebo (P<0.05). In contrast, the same ointment containing hydrocortisone alone and other ointments containing steroids or local anesthetics had no effects. DISCUSSION: Treatment with 1 mg/kg NG-nitro-L-arginine methyl ester HCl (L-NAME), a non-selective nitric oxide synthase inhibitor, significantly suppressed the effect of BCS+HC ointment (160 mg/kg) in lowering the internal anal pressure. Furthermore, BCS+HC ointment (160 mg/kg) significantly lowered capsaicin-induced high internal anal pressure compared to a placebo. CONCLUSION: These findings suggest that BCS+HC and BCS ointments containing an E. coli culture suspension significantly lowered the internal anal pressure due to endogenous nitric oxide production in conscious rats. |
| | |
Authors:
|
Hiroko Kido; Hiroshi Yasukawa; Tsuyoshi Hirota; Akihiro Shindo; Tomohiro Naruse |
Related Documents
:
|
222559 - Effects of endogenous hyperglucagonemia on lower esophageal sphincter pressure and gast... 9102269 - Is colonic electrical activity a similar phenomena to small-bowel electrical activity? 11490379 - Development of a sleeve sensor for measurement of sphincter of oddi motility. 1624149 - Oesophageal propulsive force and its relation to manometric pressure. 15715529 - Effect of vaginal distension on anorectal function: identification of the vagino-anorec... 17608849 - Assessment of esophageal motor function using combined multichannel intraluminal impeda... 9352699 - Comparison of tamsulosin with alfuzosin in the treatment of patients with lower urinary... 18496289 - Is blood pressure during the night more predictive of cardiovascular outcome than durin... 20345789 - The longpen--the world's first original remote signing device. |
Publication Detail:
|
Type: Journal Article Date: 2006-04-21 |
Journal Detail:
|
Title: International journal of colorectal disease Volume: 22 ISSN: 0179-1958 ISO Abbreviation: Int J Colorectal Dis Publication Date: 2007 Mar |
Date Detail:
|
Created Date: 2007-01-11 Completed Date: 2007-11-27 Revised Date: - |
Medline Journal Info:
|
Nlm Unique ID: 8607899 Medline TA: Int J Colorectal Dis Country: Germany |
Other Details:
|
Languages: eng Pagination: 271-6 Citation Subset: IM |
Affiliation:
|
Research Laboratories, Kyoto R&D Center, Maruho Co. Ltd., 93 Awata-cho, Chudoji, Shimogyo-ku, 600-8815, Kyoto, Japan. kido_afd@mii.maruho.co.jp |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Administration, Topical Anal Canal / drug effects* Animals Escherichia coli* Glucocorticoids / administration & dosage* Hydrocortisone / administration & dosage* Male Manometry Models, Animal Nitric Oxide / biosynthesis Ointments / administration & dosage Pressure Rats Rats, Sprague-Dawley |
| Chemical | |
Reg. No./Substance:
|
0/Glucocorticoids; 0/Ointments; 10102-43-9/Nitric Oxide; 50-23-7/Hydrocortisone |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Molecularly imprinted polymers applied to the clean-up of zearalenone and alpha-zearalenol from cere...
Next Document: Identification of neurons that express 5-hydroxytryptamine4 receptors in intestine.