Document Detail


Decrease in the linking number of plasmid DNA in dnaA mutants of Escherichia coli.
MedLine Citation:
PMID:  8573119     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
We made use of agarose gel electrophoresis in the presence of chloroquine to examine the linking number of plasmids in temperature-sensitive dnaA mutants, including dnaA5 and dnaA46 mutants. The linking number of DNA prepared from dnaA mutants growing at 37 degrees C was lower than that from wild type cells yet there was no significant difference when cells were grown at 28 degrees C. Complementation analysis with a plasmid containing the wild type dnaA gene and phage P1-mediated transduction confirmed that mutations in the dnaA gene were responsible for the decrease in the linking number of DNA.
Authors:
T Mizushima; J Tanino; T Miki; K Sekimizu
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Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Biochemical and biophysical research communications     Volume:  218     ISSN:  0006-291X     ISO Abbreviation:  Biochem. Biophys. Res. Commun.     Publication Date:  1996 Jan 
Date Detail:
Created Date:  1996-03-01     Completed Date:  1996-03-01     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0372516     Medline TA:  Biochem Biophys Res Commun     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  137-41     Citation Subset:  IM    
Affiliation:
Faculty of Pharmaceutical Sciences, Kyushu University, Fukuoka, Japan.
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MeSH Terms
Descriptor/Qualifier:
Bacterial Proteins / genetics*
DNA-Binding Proteins / genetics*
Escherichia coli / genetics*
Genes, Bacterial
Genetic Complementation Test
Mutation*
Plasmids / analysis,  isolation & purification,  metabolism*
Species Specificity
Chemical
Reg. No./Substance:
0/Bacterial Proteins; 0/DNA-Binding Proteins; 0/DnaA protein, Bacteria

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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