| Decrease in fibrin content of venous thrombi in selectin-deficient mice. | |
| | |
MedLine Citation:
|
PMID: 12591228 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
The purpose of this study was to quantify the fibrin content of thrombi produced in a mouse model of venous thrombosis and correlate this to thrombus mass. The role of P-selectin, E-selectin, and IL-10 on thrombus fibrin content was analyzed using knockout (KO) mice. Five groups of mice were evaluated: control (N = 10), P-selectin KO (N = 7), E-selectin KO (N = 5), combined E-/P-selectin KO (N = 12), and IL-10 KO (N = 10). Venous thrombosis was induced by ligation of the infrarenal IVC. Mice were sacrificed on postoperative days (POD) 2 and 6. Thrombus mass was calculated. Sections of IVC were stained with an antibody that cross reacts with mouse fibrin. The distribution of RGB color pixels was generated from digitized micrographs of the thrombus of each animal. The mean pixel value for each group was compiled and analyzed using 2-way ANOVA. Mean pixel value per group was correlated with the mean thrombus mass per group. Color analysis demonstrated significant decreases in the analyzed fibrin content on POD-2 between the control vs E-/P-selectin KO (P < 0.05) and control vs IL-10 KO (P < 0.05) groups. In addition, significantly less fibrin staining was noted on POD-6 between the control vs E-selectin KO (P = 0.03), control vs P-selectin KO (P = 0.01), and control vs E-/P-selectin KO (P < 0.01). There was a strong overall correlation between the mean pixel value for each group and the thrombus mass (R = 0.964; P < 0.01). This study demonstrates a difference in fibrin content of thrombi produced in animals deficient in E-selectin, P-selectin, and IL-10, supporting their importance in thrombus amplification, fibrin formation, and the mass of thrombus formed. |
| | |
Authors:
|
V V Sullivan; A E Hawley; D M Farris; B S Knipp; A J Varga; S K Wrobleski; P Thanapron; M J Eagleton; D D Myers; J B Fowlkes; T W Wakefield |
Related Documents
:
|
17270288 - Exploratory activity, motor coordination, and spatial learning in mchr1 knockout mice. 17679618 - Soluble guanylate cyclase-alpha1 deficiency selectively inhibits the pulmonary vasodila... 16943298 - Acid sphingomyelinase deficiency increases susceptibility to fatal alphavirus encephalo... 9922208 - Proliferation and differentiation defects during lung development in corticotropin-rele... 1655638 - Impairment of cytokine production in mice fed a vitamin d3-deficient diet. 14625098 - Serum cytokine levels in patients with acute brucellosis and their relation to the trad... |
Publication Detail:
|
Type: Journal Article; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
|
Title: The Journal of surgical research Volume: 109 ISSN: 0022-4804 ISO Abbreviation: J. Surg. Res. Publication Date: 2003 Jan |
Date Detail:
|
Created Date: 2003-02-19 Completed Date: 2003-03-07 Revised Date: 2009-11-03 |
Medline Journal Info:
|
Nlm Unique ID: 0376340 Medline TA: J Surg Res Country: United States |
Other Details:
|
Languages: eng Pagination: 1-7 Citation Subset: IM |
Affiliation:
|
Jobst Vascular Research Laboratories, University of Michigan, Department of Surgery, Section of Vascular Surgery, Ann Arbor, Michigan 48109, USA. vitas@umich.edu |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Animals E-Selectin / genetics, physiology* Fibrin / analysis* Immunohistochemistry Interleukin-10 / deficiency, genetics, physiology Leukocyte Count Lymphocyte Count Mice Mice, Knockout Microscopy Monocytes Neutrophils P-Selectin / genetics, physiology* Time Factors Venous Thrombosis / metabolism*, pathology |
| Grant Support | |
ID/Acronym/Agency:
|
R01 63148//PHS HHS |
| Chemical | |
Reg. No./Substance:
|
0/E-Selectin; 0/P-Selectin; 130068-27-8/Interleukin-10; 9001-31-4/Fibrin |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Epinephrine enhancement of human memory consolidation: interaction with arousal at encoding.
Next Document: Novel protection strategy for pulmonary transplantation.