Document Detail

Decrease in core liver temperature with 10 degrees C by in situ hypothermic perfusion under total hepatic vascular exclusion reduces liver ischemia and reperfusion injury during partial hepatectomy in pigs.
MedLine Citation:
PMID:  14639360     Owner:  NLM     Status:  MEDLINE    
OBJECTIVE: We attempted to assess liver ischemia/reperfusion injury under a mild decrease in core liver temperature of 10 degrees C by in situ hypothermic perfusion during ischemia. METHODS: Liver ischemia was induced in pigs by total hepatic vascular exclusion with concomitant in situ perfusion with hypothermic (4 degrees C) Ringer-glucose (cold perfused group, core liver temperature maintained at 28 degrees C), with normothermic (38 degrees C) Ringer-glucose (warm perfused group) or without in situ perfusion (control group). RESULTS: In the cold perfused, warm perfused, and control groups, 24-hour survival was 5/5, 0/5, and 3/5, respectively. Hemodynamic parameters in the cold perfused group remained stable, whereas pigs in both other groups required circulatory support. Plasma AST and interleukin-6 levels were lower in the cold perfused group than in both other groups. Hepatocellular function was best preserved in the cold perfused group as indicated by complete recovery of bile production during reperfusion and no loss of indocyanine green clearance capacity. In both other groups, bile production and indocyanine green clearance capacity were reduced significantly. The hyaluronic acid uptake capacity of pigs in the cold perfused group or control group did not differ, indicating preserved sinusoidal endothelial cell function. Histopathologic injury scores during reperfusion were significantly lower in the cold perfused group when compared to both other groups. CONCLUSIONS: A mild decrease in core liver temperature of 10 degrees C by in situ hypothermic liver perfusion during ischemia protects the liver from ischemia/reperfusion injury. This protection appears to be related to cooling of the liver rather than to the washout of blood during perfusion.
Bob H M Heijnen; Irene H Straatsburg; Dirk J Gouma; Thomas M van Gulik
Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Surgery     Volume:  134     ISSN:  0039-6060     ISO Abbreviation:  Surgery     Publication Date:  2003 Nov 
Date Detail:
Created Date:  2003-11-25     Completed Date:  2004-01-05     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0417347     Medline TA:  Surgery     Country:  United States    
Other Details:
Languages:  eng     Pagination:  806-17     Citation Subset:  AIM; IM    
Surgical laboratory, IWO 10151, Academic Medical Center, Meibergdreef 9, 1105 AZ Amsterdam, The Netherlands.
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MeSH Terms
Body Temperature
Endothelial Cells / physiology
Hypothermia, Induced*
Interleukin-6 / blood
Ischemia / prevention & control*
Liver / blood supply*,  pathology
Liver Circulation*
Reperfusion Injury / prevention & control*
Reg. No./Substance:

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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