Document Detail

Decrease in cell viability in an RMF, sigma(38), and OmpC triple mutant of Escherichia coli.
MedLine Citation:
PMID:  12437978     Owner:  NLM     Status:  MEDLINE    
In a speG-disrupted Escherichia coli mutant, which cannot metabolize spermidine to acetylspermidine, addition of spermidine to the medium caused a decrease in cell viability at the late stationary phase of growth. There were parallel decreases in the levels of ribosome modulation factor (RMF), the sigma(38) subunit of RNA polymerase, and the outer membrane protein C (OmpC). To clarify that these three proteins are strongly involved in cell viability, the rmf, rpoS (encoding sigma(38)), and ompC genes were disrupted. Viability of the triple mutant decreased to less than 1% of normal cells. The triple mutant had a reduced cell viability compared to any combination of double mutants, which also had a reduced cell viability. The single rmf and rpoS, but not ompC, mutant only slightly reduced cell viability. The results indicate that cooperative functions of these three proteins are necessary for cell viability at the late stationary phase. The triple mutant had a reduced level of ribosomes and of intracellular cations.
V Samuel Raj; Christine Füll; Madoka Yoshida; Kaori Sakata; Keiko Kashiwagi; Akira Ishihama; Kazuei Igarashi
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Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Biochemical and biophysical research communications     Volume:  299     ISSN:  0006-291X     ISO Abbreviation:  Biochem. Biophys. Res. Commun.     Publication Date:  2002 Nov 
Date Detail:
Created Date:  2002-11-19     Completed Date:  2003-01-09     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0372516     Medline TA:  Biochem Biophys Res Commun     Country:  United States    
Other Details:
Languages:  eng     Pagination:  252-7     Citation Subset:  IM    
Graduate School of Pharmaceutical Sciences, Chiba University, 1-33 Yayoi-cho, Inage-ku, 263-8522, Chiba, Japan.
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MeSH Terms
Bacterial Proteins / genetics,  physiology*
Cell Division
Centrifugation, Density Gradient
Escherichia coli / cytology,  genetics,  metabolism*
Escherichia coli Proteins / genetics,  physiology*
Porins / genetics,  physiology*
Ribosomal Proteins / genetics,  physiology*
Ribosomes / metabolism
Sigma Factor / genetics,  physiology*
Spermidine / pharmacology
Reg. No./Substance:
0/Bacterial Proteins; 0/Escherichia coli Proteins; 0/OmpC protein; 0/Porins; 0/Ribosomal Proteins; 0/Sigma Factor; 0/ribosome modulation factor, E coli; 0/sigma factor KatF protein, Bacteria; 124-20-9/Spermidine

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