| Decrease in calcium-sensing receptor in the progress of diabetic cardiomyopathy. | |
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MedLine Citation:
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PMID: 22137362 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
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To observe the dynamic expression of calcium-sensing receptor (CaSR) in myocardium of diabetic rats and explore its role in diabetic cardiomyopathy (DCM), 40 male Wistar rats were randomly divided into 4 groups including control, diabetic-4 weeks, diabetic-8 weeks and spermine treatment groups (240μM of spermine in drinking water). The type 2 Diabetes mellitus (DM) models were established by intraperitoneal injection of streptozotocin (STZ, 30mg/kg) after high-fat and high-sugar diet for one month. The echocardiographic parameters were measured, cardiac morphology was observed by electron microscope and HE staining. The intracellular calcium concentration ([Ca(2+)](i)) was detected by laser-scanning confocal microscope. Western blot analyzed the expression of CaSR, protein kinase C α(PKC-α) and calcium handling regulators, such as phospholamban (PLN), Ca(2+)-ATPase (SERCA), and ryanodine receptor (RyR). Compared with control group, [Ca(2+)](i) and the expression of CaSR, RyR and SERCA/PLN were decreased, while PKC-α and PLN were significantly increased in a time-dependent manner in diabetic groups. Meanwhile diabetic rats displayed abnormal cardiac structure and systolic and diastolic dysfunction, and spermine (CaSR agonist) could prevent or slow its progression. These results indicate that the CaSR expression of myocardium is reduced in the progress of DCM, and its potential mechanism is related to the impaired intracellular calcium homeostasis. |
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Authors:
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Shu-Zhi Bai; Jian Sun; Hao Wu; Ning Zhang; Hong-Xia Li; Guang-Wei Li; Hong-Zhu Li; Wen He; Wei-Hua Zhang; Ya-Jun Zhao; Li-Na Wang; Ye Tian; Bao-Feng Yang; Guang-Dong Yang; Ling-Yun Wu; Rui Wang; Chang-Qing Xu |
Publication Detail:
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Type: JOURNAL ARTICLE Date: 2011-12-1 |
Journal Detail:
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Title: Diabetes research and clinical practice Volume: - ISSN: 1872-8227 ISO Abbreviation: - Publication Date: 2011 Dec |
Date Detail:
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Created Date: 2011-12-5 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 8508335 Medline TA: Diabetes Res Clin Pract Country: - |
Other Details:
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Languages: ENG Pagination: - Citation Subset: - |
Copyright Information:
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Copyright © 2011 Elsevier Ireland Ltd. All rights reserved. |
Affiliation:
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Department of Pathophysiology, Harbin Medical University, Harbin 150086, China. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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