| A Decrease in DKK1, a WNT Inhibitor, Contributes to Placental Lipid Accumulation in an Obesity-Prone Rat Model. | |
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MedLine Citation:
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PMID: 22133691 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
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Placenta, as the sole transport mechanism between mother and fetus, links the maternal physical state and the immediate and life-long outcomes of the offspring. The present study examined the consequences of maternal obesity on placental lipid accumulation and metabolism. Pregnant obesity prone (OP) and obesity resistant (OR) rat strains were fed a control diet throughout gestation. Placentas were collected on Gestational Day 21 for mRNA and oxidative stress analysis and frozen placental sections were analyzed for fat accumulation as well as beta-catenin and DKK1 localization. JEG3 trophoblast cells were cultured in vitro to determine the relationship between DKK1 and lipid accumulation. Maternal plasma and placental NEFA and TG were elevated in OP dams. Placental Dkk1 mRNA content was 4-fold lower in OP placentas, and there was a significant increase in beta-catenin accumulation as well as mRNA content of fat transport and TG synthesis genes, including Ppard, Slc27a1 (Fatp1), Cd36 (Fat), Lipin1, and Lipin3. There was significant lipid accumulation within the decidual zones in OP but not OR placentas, and the thickness of the decidual and junctional zones was significantly smaller in OP than OR placentas. Overexpression of DKK1 in JEG3 cells decreased lipid accumulation and the mRNA content of PPARD, SLC27A1, CD36, LIPIN1, and LIPIN3. Our results demonstrate that DKK1 is regulating certain aspects of placental lipid metabolism through the WNT signaling pathway. |
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Authors:
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Rita S Strakovsky; Yuan-Xiang Pan |
Publication Detail:
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Type: JOURNAL ARTICLE Date: 2011-11-30 |
Journal Detail:
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Title: Biology of reproduction Volume: - ISSN: 1529-7268 ISO Abbreviation: - Publication Date: 2011 Nov |
Date Detail:
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Created Date: 2011-12-2 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 0207224 Medline TA: Biol Reprod Country: - |
Other Details:
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Languages: ENG Pagination: - Citation Subset: - |
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