Document Detail


Deconvolution as a novel approach to analyze moment-to-moment free fatty acid release.
MedLine Citation:
PMID:  17925467     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
OBJECTIVE: Recent studies have shown that free fatty acid (FFA) release is pulsatile and that this pattern is controlled by the sympathetic nervous system. It is, then, necessary to understand and characterize adipose tissue lipolysis to elucidate its effect on metabolism. In this study, we introduce deconvolution as a method to detect and quantify pulsatile FFA release. RESEARCH METHODS AND PROCEDURES: Octanoate, a medium-chain fatty acid, was infused in male mongrel dogs (n = 7) to mimic the pulsatile appearance of plasma FFAs. Deconvolution analysis was used to reconstruct the number and timing of infused octanoate pulses from plasma FFA concentrations. RESULTS: Deconvolution analysis was able to reconstruct the exogenously infused pulses of octanoate used to mimic pulsatile appearance of FFAs (pulse frequency, 8 per hour; interpulse interval, 7 minutes). However, determination of pulse mass was less accurate (1.0 +/- 0.0 vs. 0.54 +/- 0.1 mM). The addition of varying levels of Gaussian noise to non-oscillatory FFA time series did not lead to detection of extraneous FFA pulses. However, goodness of fit declined with increasing variability. DISCUSSION: These results support the use of deconvolution as an accurate approach to determine the temporal sequence of endogenous FFA release.
Authors:
Katrin Hücking; Isabel R Hsu; Viorica Ionut; Richard N Bergman
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Obesity (Silver Spring, Md.)     Volume:  15     ISSN:  1930-7381     ISO Abbreviation:  Obesity (Silver Spring)     Publication Date:  2007 Oct 
Date Detail:
Created Date:  2007-10-10     Completed Date:  2008-01-22     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  101264860     Medline TA:  Obesity (Silver Spring)     Country:  United States    
Other Details:
Languages:  eng     Pagination:  2416-23     Citation Subset:  IM    
Affiliation:
Department of Physiology and Biophysics, Keck School of Medicine, University of Southern California, 1333 San Pablo Street, Mudd Memorial Research 626, Los Angeles CA 90033, USA.
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MeSH Terms
Descriptor/Qualifier:
Animals
Dogs
Fatty Acids, Nonesterified / blood*,  metabolism*
Kinetics
Male
Octanoic Acids / blood*,  metabolism*
Grant Support
ID/Acronym/Agency:
DK27619/DK/NIDDK NIH HHS; DK29867/DK/NIDDK NIH HHS
Chemical
Reg. No./Substance:
0/Fatty Acids, Nonesterified; 0/Octanoic Acids; 124-07-2/caprylic acid

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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