Document Detail

Deconvolution as a novel approach to analyze moment-to-moment free fatty acid release.
MedLine Citation:
PMID:  17925467     Owner:  NLM     Status:  MEDLINE    
OBJECTIVE: Recent studies have shown that free fatty acid (FFA) release is pulsatile and that this pattern is controlled by the sympathetic nervous system. It is, then, necessary to understand and characterize adipose tissue lipolysis to elucidate its effect on metabolism. In this study, we introduce deconvolution as a method to detect and quantify pulsatile FFA release. RESEARCH METHODS AND PROCEDURES: Octanoate, a medium-chain fatty acid, was infused in male mongrel dogs (n = 7) to mimic the pulsatile appearance of plasma FFAs. Deconvolution analysis was used to reconstruct the number and timing of infused octanoate pulses from plasma FFA concentrations. RESULTS: Deconvolution analysis was able to reconstruct the exogenously infused pulses of octanoate used to mimic pulsatile appearance of FFAs (pulse frequency, 8 per hour; interpulse interval, 7 minutes). However, determination of pulse mass was less accurate (1.0 +/- 0.0 vs. 0.54 +/- 0.1 mM). The addition of varying levels of Gaussian noise to non-oscillatory FFA time series did not lead to detection of extraneous FFA pulses. However, goodness of fit declined with increasing variability. DISCUSSION: These results support the use of deconvolution as an accurate approach to determine the temporal sequence of endogenous FFA release.
Katrin Hücking; Isabel R Hsu; Viorica Ionut; Richard N Bergman
Related Documents :
17876057 - Whole body distribution of deuterated linoleic and alpha-linolenic acids and their meta...
11377587 - Plasma concentrations of estradiol 17beta and pgf2alpha metabolite and placental fatty ...
1917977 - Regulation of expression of the fatty acid synthase gene in 3t3-l1 cells by differentia...
22133197 - The mediterranean diet: effects on proteins that mediate fatty acid metabolism in the c...
24306687 - Amino acid uptake by lemna gibba by a mechanism with affinity to neutral l-and d-amino ...
3589497 - The role of fat and bile acid malabsorption in diarrhoea of coeliac disease.
Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Obesity (Silver Spring, Md.)     Volume:  15     ISSN:  1930-7381     ISO Abbreviation:  Obesity (Silver Spring)     Publication Date:  2007 Oct 
Date Detail:
Created Date:  2007-10-10     Completed Date:  2008-01-22     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  101264860     Medline TA:  Obesity (Silver Spring)     Country:  United States    
Other Details:
Languages:  eng     Pagination:  2416-23     Citation Subset:  IM    
Department of Physiology and Biophysics, Keck School of Medicine, University of Southern California, 1333 San Pablo Street, Mudd Memorial Research 626, Los Angeles CA 90033, USA.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Fatty Acids, Nonesterified / blood*,  metabolism*
Octanoic Acids / blood*,  metabolism*
Grant Support
Reg. No./Substance:
0/Fatty Acids, Nonesterified; 0/Octanoic Acids; 124-07-2/caprylic acid

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Previous Document:  Cardiac fas receptor-dependent apoptotic pathway in obese Zucker rats.
Next Document:  Viral mediated neuropeptide Y expression in the rat paraventricular nucleus results in obesity.