Document Detail


Deconvoluting topography and spatial physiological activity of live macrophage cells by scanning electrochemical microscopy in constant-distance mode.
MedLine Citation:
PMID:  20873801     Owner:  NLM     Status:  In-Process    
Abstract/OtherAbstract:
Detection of reactive oxygen species (ROS) released from live macrophage cells (RAW264.7) without any addition of external redox mediators using constant-height and constant-distance mode scanning electrochemical microscopy (SECM) was presented in this Letter. The successful separation of the ROS profile from the topography of cells in the physiological condition was demonstrated by recording the amperometric current and probing position in the z-direction along with lateral coordinates at each pixel where an alternating current (AC) was kept constant. It was discovered that the nucleus region of the cell releases more ROS than other organelle regions and the height of the cell is approximately 4.8 μm. To our best knowledge, this work reports the first spatially monitored ROS release without the influence of cell morphology using SECM.
Authors:
Xiaocui Zhao; Piotr M Diakowski; Zhifeng Ding
Publication Detail:
Type:  Letter; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Analytical chemistry     Volume:  82     ISSN:  1520-6882     ISO Abbreviation:  Anal. Chem.     Publication Date:  2010 Oct 
Date Detail:
Created Date:  2011-04-15     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0370536     Medline TA:  Anal Chem     Country:  United States    
Other Details:
Languages:  eng     Pagination:  8371-3     Citation Subset:  IM    
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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