Document Detail


Decolorization of azo dyes by Geobacter metallireducens.
MedLine Citation:
PMID:  23132348     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
Geobacter metallireducens was found to be capable of decolorizing several azo dyes with different structures to various extents. Pyruvate, ethanol, acetate, propionate, and benzoate could support 66.3 ± 2.6-93.7 ± 2.1 % decolorization of 0.1 mM acid red 27 (AR27) in 40 h. The dependence of the specific decolorization rate on AR27 concentration (25 to 800 μM) followed Michaelis-Menten kinetics (K (m) = 186.9 ± 1.4 μΜ, V (max) = 0.65 ± 0.02 μmol mg protein(-1) h(-1)). Enhanced AR27 decolorization was observed with the increase of cell concentrations ranging from 7.5 to 45 mgL(-1). AR27 decolorization by G. metallireducens was retarded by the presence of goethite, which competed electrons with AR27 and was reduced to Fe(II). The addition of low concentrations of humic acid (1-100 mgL(-1)) or 2-hydroxy-1,4-naphthoquinone (0.5-50 μM) could improve the decolorization performance of G. metallireducens. High-performance liquid chromatography analysis suggested reductive pathway to be responsible for decolorization. This was the first study on azo dye decolorization by Geobacter strain and might improve our understanding of natural attenuation and bioremediation of environments polluted by azo dyes.
Authors:
Guangfei Liu; Jiti Zhou; Congcong Chen; Jing Wang; Ruofei Jin; Hong Lv
Related Documents :
8135378 - High-resolution tandem mass spectrometry of carbonic anhydrase.
16908038 - Primary structure of a thrombin-like serine protease, kangshuanmei, from the venom of a...
23157008 - Efficient counter-current chromatographic isolation and structural identification of tw...
23063698 - Screening of lactic acid bacteria for reducing power using a tetrazolium salt reduction...
8135378 - High-resolution tandem mass spectrometry of carbonic anhydrase.
4058 - Adsorption of glyceraldehyde 3-phosphate dehydrogenase on condensed monolayers of phosp...
Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2012-11-8
Journal Detail:
Title:  Applied microbiology and biotechnology     Volume:  -     ISSN:  1432-0614     ISO Abbreviation:  Appl. Microbiol. Biotechnol.     Publication Date:  2012 Nov 
Date Detail:
Created Date:  2012-11-7     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8406612     Medline TA:  Appl Microbiol Biotechnol     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Affiliation:
Key Laboratory of Industrial Ecology and Environmental Engineering (Ministry of Education), School of Environmental Science and Dalian University of Technology, Dalian, 116024, Republic of China.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Characterization of recombinant amylopullulanase (gt-apu) and truncated amylopullulanase (gt-apuT) o...
Next Document:  Recurrence of paroxysmal atrial fibrillation after cryoisolation of the pulmonary veins. Is a "redo"...