Document Detail

Decidualization of human endometrial stromal cells in vitro: effects of progestin and relaxin on the ultrastructure and production of decidual secretory proteins.
MedLine Citation:
PMID:  7517949     Owner:  NLM     Status:  MEDLINE    
Decidual cells arise by proliferation and differentiation of endometrial stromal cells of the uterus after appropriate stimulation by ovarian hormones. Previously we have shown that progestin and relaxin stimulate the secretion of several decidual-cell-specific secretory proteins in a long-term primary cell culture system. We now report the effects of progestin and relaxin on the morphology of stromal cells in association with the production rate of two major decidual secretory proteins, prolactin and insulin-like growth factor binding protein-1 (IGFBP-1). Stromal cells were cultured in RPMI 1640 and 2% fetal bovine serum for 22 days under control conditions (no hormone), with relaxin or medroxyprogesterone acetate (MPA), or MPA plus relaxin. Cells treated with MPA alone or MPA plus porcine relaxin grew to a high density with many areas of heaping while control cells and cells grown in medium containing relaxin alone formed discontinuous layers. The cytoplasm was distinguished by aggregates of rough endoplasmic reticulum and secretory granules. Surfaces of cells treated with MPA plus relaxin had clusters of short blunt processes containing secretory granules. The processes were rarely seen in cells exposed to MPA alone and completely absent in control cells or cells exposed to relaxin alone. Intercellular space was greatly widened in cells treated with MPA alone or MPA plus relaxin. There were many extended gap junctions in MPA- and relaxin-treated cells in contrast to controls.(ABSTRACT TRUNCATED AT 250 WORDS)
B Lane; W Oxberry; J Mazella; L Tseng
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Human reproduction (Oxford, England)     Volume:  9     ISSN:  0268-1161     ISO Abbreviation:  Hum. Reprod.     Publication Date:  1994 Feb 
Date Detail:
Created Date:  1994-08-09     Completed Date:  1994-08-09     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  8701199     Medline TA:  Hum Reprod     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  259-66     Citation Subset:  IM    
Department of Pathology, State University of New York at Stony Brook 11794.
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MeSH Terms
Carrier Proteins / biosynthesis*,  ultrastructure
Cells, Cultured
Decidua / cytology,  drug effects*,  physiology
Insulin-Like Growth Factor Binding Protein 1
Microscopy, Electron
Middle Aged
Progestins / pharmacology*
Prolactin / biosynthesis*
Relaxin / pharmacology*
Somatomedins / biosynthesis*,  ultrastructure
Stromal Cells / drug effects,  physiology,  ultrastructure
Grant Support
Reg. No./Substance:
0/Carrier Proteins; 0/Insulin-Like Growth Factor Binding Protein 1; 0/Progestins; 0/Somatomedins; 9002-62-4/Prolactin; 9002-69-1/Relaxin

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