Document Detail


Decidual sialylation shows species-specific differences in the pregnant mouse and rat.
MedLine Citation:
PMID:  8699407     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Biotinylated lectins from Sambucus nigra (SNA) and Maackia amurensis (MAA), which bind to alpha 2,6-linked and alpha 2,3-linked sialyl residues, respectively, were used as probes to study glycan terminal modifications associated with decidualization in the uterine stroma of pregnant rats and mice. Binding of lectins from Erythrina cristagalli (ECA), Phaseolus vulgaris (leukoagglutinin, L-PHA), Triticum vulgaris (WGA) and Bandeiraea simplicifolia (BSA-1B4) was also examined. Tissues from rats between day 5 and day 8 of gestation and mice between day 5 and day 7 of gestation were fixed in Bouin's solution and embedded in wax prior to lectin histochemistry. On day 7 in rats and day 6 in mice, there was a marked reduction in the binding of SNA in the subluminal decidua surrounding the implantation site. In rats, MAA binding to enlarged decidual cells around the implantation chamber was increased markedly, but there was no change in mice. In both species there was de novo binding of ECA in the SNA-negative area, suggesting that the loss of alpha 2,6-linked sialyl residues unmasks terminal N-acetyl lactosamine. These findings are consistent with previous evidence of a close structural and functional similarity between the artificially induced deciduoma and true decidua of rats and show identical changes to the glycosylation patterns previously found in differentiating rat deciduoma. In both species, therefore, decidua exhibits regionally specific terminal glycosylation. However, the species-specific expression of alpha 2,3-linked sialyl residues suggests distinct patterns of steroidally modulated sialyl transferase expression.
Authors:
C J Jones; S J Kimber; I Illingworth; J D Aplin
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Journal of reproduction and fertility     Volume:  106     ISSN:  0022-4251     ISO Abbreviation:  J. Reprod. Fertil.     Publication Date:  1996 Mar 
Date Detail:
Created Date:  1996-09-05     Completed Date:  1996-09-05     Revised Date:  2007-11-15    
Medline Journal Info:
Nlm Unique ID:  0376367     Medline TA:  J Reprod Fertil     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  241-50     Citation Subset:  IM    
Affiliation:
Department of Pathological Sciences, University of Manchester, UK.
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MeSH Terms
Descriptor/Qualifier:
Animals
Decidua / growth & development,  metabolism*
Erythrina
Female
Gestational Age
Glycosylation
Histocytochemistry
Lectins / metabolism
Mice
Mice, Inbred Strains
Phytohemagglutinins / metabolism
Plant Lectins
Plants, Medicinal
Pregnancy
Rats
Rats, Sprague-Dawley
Ribosome Inactivating Proteins
Sialyltransferases / metabolism*
Species Specificity
Time Factors
Chemical
Reg. No./Substance:
0/Griffonia simplicifolia lectins; 0/Lectins; 0/Phytohemagglutinins; 0/Plant Lectins; 0/Sambucus nigra lectins; 0/erythrina lectin; 0/leukoagglutinins, plants; EC 2.4.99.-/Sialyltransferases; EC 3.2.2.22/Ribosome Inactivating Proteins

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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