| Decellularized native and engineered arterial scaffolds for transplantation. | |
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MedLine Citation:
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PMID: 14579934 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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More than 570,000 coronary artery bypass grafts are implanted each year, creating an important demand for small-diameter vascular grafts. For patients who lack adequate internal mammary artery or saphenous vein, tissue-engineered arteries may prove useful. However, the time needed to tissue engineer arteries (7 weeks or more) is too long for many patients. Decellularized cadaveric human arteries are another possible source of vascular conduit, but limited availability and the potential for disease transmission limit their widespread use. In contrast, decellularized tissue-engineered arteries could serve as grafts for immediate implantation, as scaffolds onto which patients' cells could be seeded, or as carriers for genetically engineered cells to aid cell transplantation. The goal of this study was to quantify the effects of decellularization on vascular matrix and mechanical properties. Specifically, we compared cellular elimination, extracellular matrix retention, and mechanical characteristics of porcine carotid arteries before and after treatment with three decellularization methods. In addition, for the first time, tissue-engineered arteries were decellularized. Decellularized native arteries were also used as a scaffold onto which vascular cells were seeded. These studies identified a decellularization method for native and engineered arteries that maximized cellular elimination, without greatly compromising mechanical integrity. We showed that engineered tissues could be decellularized, and demonstrated the feasibility of reseeding decellularized vessels with vascular cells. |
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Authors:
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Shannon L M Dahl; Jennifer Koh; Vikas Prabhakar; Laura E Niklason |
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Publication Detail:
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Type: Comparative Study; Journal Article; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
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Title: Cell transplantation Volume: 12 ISSN: 0963-6897 ISO Abbreviation: Cell Transplant Publication Date: 2003 |
Date Detail:
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Created Date: 2003-10-28 Completed Date: 2004-04-08 Revised Date: 2007-11-14 |
Medline Journal Info:
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Nlm Unique ID: 9208854 Medline TA: Cell Transplant Country: United States |
Other Details:
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Languages: eng Pagination: 659-66 Citation Subset: IM |
Affiliation:
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Department of Biomedical Engineering, Duke University, Durham, NC 27708, USA. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Artificial Organs / trends Biocompatible Materials / pharmacology*, therapeutic use Carotid Arteries / cytology, immunology, transplantation* Cattle Cell Separation / methods* Collagen / metabolism Coronary Artery Bypass / methods DNA / metabolism Extracellular Matrix / immunology, metabolism Humans Muscle, Smooth, Vascular / cytology, physiology, transplantation Stress, Mechanical Sus scrofa Tissue Engineering / methods* Tissue Transplantation / methods* Transplantation, Heterologous / methods* Weight-Bearing / physiology |
| Grant Support | |
ID/Acronym/Agency:
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HL63766/HL/NHLBI NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Biocompatible Materials; 9007-34-5/Collagen; 9007-49-2/DNA |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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