Document Detail

Decellularization as an anticalcification method in stentless bovine pericardium valve prosthesis: a study in sheep.
MedLine Citation:
PMID:  22086579     Owner:  NLM     Status:  Publisher    
OBJECTIVE: The objective was to analyze the decellularization process with SDS in glutaraldehyde-preserved bovine pericardium as an anticalcification method in a circulatory sheep model. METHODS: The valved tubs were implanted in pulmonary artery position in sheep by 180 days. The animals were divided in two groups of 8 animals: control group glutaraldehyde-preserved bovine pericardium and the study group - decellularized bovine pericardium with 0,1% SDS and glutaraldehyde-preserved. After explantation the tubs were analized by x-ray macroscopy, hematoxilin-eosin, alizarin-red and Russel-Movatz pentacromic histology. The calcium content was measured by flame atomic absorption spectrometry. RESULTS: There was no early mortality, but two animals in each group died during the study. All cusps in the control group were severely calcified and in some points in the conduits, while the decellularized group did not show macroscopic calcification. Data were proved by x-ray and histologycal exams. The matrix was preserved in histologycal analysis in decellularized group, without gross calcification. The wall conduits calcium content was 35,25±42,13 µg/mg in the control group versus 15,75±10,44 µg/mg in the decellularized one: in the cusps was 264,4±126,16 µg/mg in control group versus 94,29±27,05 µg/mg in decellularized group (P=0,009). CONCLUSION: The decellularization with 0.1% SDS was effective as an anticalcification method in bovine pericardial grafts implanted in a sheep circulatory model for 180 days.
Claudinei Collatusso; João Gabriel Roderjan; Eduardo Discher Vieira; Nelson Itiro Myague; Lúcia de Noronha; Francisco Diniz Affonso da Costa
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Publication Detail:
Journal Detail:
Title:  Revista brasileira de cirurgia cardiovascular : orgao oficial da Sociedade Brasileira de Cirurgia Cardiovascular     Volume:  26     ISSN:  1678-9741     ISO Abbreviation:  -     Publication Date:  2011 Sep 
Date Detail:
Created Date:  2011-11-16     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9104279     Medline TA:  Rev Bras Cir Cardiovasc     Country:  -    
Other Details:
Languages:  ENG; POR     Pagination:  419-426     Citation Subset:  -    
Santa Casa de Curitiba, Curitiba, PR, Brasil.
Vernacular Title:
Descelularização como método anticalcificante em próteses valvares de pericárdio bovino sem suporte: estudo em ovinos.
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