Document Detail


Decalpenic acid, a novel small molecule from Penicillium verruculosum CR37010, induces early osteoblastic markers in pluripotent mesenchymal cells.
MedLine Citation:
PMID:  20940725     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Osteoblasts are the cells responsible for bone formation during embryonic development and adult life. Small compounds that could induce osteoblast differentiation might be promising sources of therapies for bone diseases such as osteoporosis. During screening for inducers of osteoblast differentiation of mouse pluripotent mesenchymal C3H10T1/2 cells, we isolated a small compound from the fermentation broth of Penicillium verruculosum CR37010. This compound, named decalpenic acid, bears a decalin moiety with a tetraenoic acid side chain. Treatment of C3H10T1/2 cells with decalpenic acid alone induced the expression of early osteoblast markers, such as alkaline phosphatase activity and osteopontin mRNA, but did not induce the late osteoblast marker osteocalcin mRNA or adipocyte markers under our experimental conditions.
Authors:
Shuichi Sakamoto; Fukiko Kojima; Masayuki Igarashi; Ryuichi Sawa; Maya Umekita; Yumiko Kubota; Koichi Nakae; Shoichi Yamaguchi; Hayamitsu Adachi; Yoshio Nishimura; Yuzuru Akamatsu
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2010-10-13
Journal Detail:
Title:  The Journal of antibiotics     Volume:  63     ISSN:  0021-8820     ISO Abbreviation:  J. Antibiot.     Publication Date:  2010 Dec 
Date Detail:
Created Date:  2010-12-23     Completed Date:  2011-01-28     Revised Date:  2012-09-06    
Medline Journal Info:
Nlm Unique ID:  0151115     Medline TA:  J Antibiot (Tokyo)     Country:  Japan    
Other Details:
Languages:  eng     Pagination:  703-8     Citation Subset:  IM    
Affiliation:
Institute of Microbial Chemistry, Shinagawa-ku, Tokyo, Japan. sakamotos@bikaken.or.jp
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MeSH Terms
Descriptor/Qualifier:
Alkaline Phosphatase / genetics,  metabolism
Animals
Carboxylic Acids / chemistry,  isolation & purification*,  pharmacology
Cell Differentiation / drug effects
Cell Line
DNA / chemistry,  genetics
Macrolides / chemistry,  isolation & purification*,  pharmacology
Mesenchymal Stem Cells / cytology,  drug effects*,  metabolism
Mice
Molecular Structure
Naphthalenes / chemistry,  isolation & purification*,  pharmacology
Nuclear Magnetic Resonance, Biomolecular
Osteoblasts / cytology,  drug effects*,  metabolism
Penicillium / chemistry*
Pluripotent Stem Cells / cytology,  drug effects*,  metabolism
Polymerase Chain Reaction
Spectrometry, Mass, Electrospray Ionization
Spectrophotometry, Ultraviolet
Chemical
Reg. No./Substance:
0/Carboxylic Acids; 0/Macrolides; 0/Naphthalenes; 0/decalpenic acid; 9007-49-2/DNA; EC 3.1.3.1/Alkaline Phosphatase

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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