Document Detail

Death mechanisms in cultured cells infected by Semliki Forest virus.
MedLine Citation:
PMID:  9225029     Owner:  NLM     Status:  MEDLINE    
We have investigated the induction of cell death in cultured cells by the virulent SFV4 and avirulent A7 strains of Semliki Forest virus (SFV). In BHK cells, death occurred by a typical apoptotic mechanism, as did the death of oligodendrocytes in glial cell cultures. For cerebellar neuron cultures, virus-induced death was due to necrosis. Although the SFV4 and A7 strains did not differ in the mechanism of induction of cell death, the virulent SFV4 strain did multiply to a higher titre in cultured neurons than the avirulent A7 strain. This is consistent with previous animal studies which indicate that the virulence of SFV strains is controlled by rapidity of multiplication in the CNS, leading to a lethal threshold of damage, rather than differential cell tropism or cell death mechanisms. The immune-mediated demyelination induced by avirulent strains may be triggered by apoptosis of oligodendrocytes, the consequences of which are obscured by death for virulent strains.
G M Glasgow; M M McGee; B J Sheahan; G J Atkins
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  The Journal of general virology     Volume:  78 ( Pt 7)     ISSN:  0022-1317     ISO Abbreviation:  J. Gen. Virol.     Publication Date:  1997 Jul 
Date Detail:
Created Date:  1997-08-12     Completed Date:  1997-08-12     Revised Date:  2009-09-29    
Medline Journal Info:
Nlm Unique ID:  0077340     Medline TA:  J Gen Virol     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  1559-63     Citation Subset:  IM    
Department of Microbiology, Moyne Institute of Preventive Medicine, Trinity College, Dublin, Ireland.
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MeSH Terms
Cell Death*
Cell Line
Cell Survival
Cells, Cultured
DNA Fragmentation
Neuroglia / cytology,  virology
Neurons / cytology,  virology
Oligodendroglia / cytology,  virology
Semliki forest virus / growth & development,  isolation & purification,  pathogenicity*
Uridine / pharmacokinetics
Grant Support
//Wellcome Trust
Reg. No./Substance:

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