Document Detail

Death-associated protein kinase 2 is a new calcium/calmodulin-dependent protein kinase that signals apoptosis through its catalytic activity.
MedLine Citation:
PMID:  10376525     Owner:  NLM     Status:  MEDLINE    
We have identified and characterized a new calcium/calmodulin (Ca2+/CaM) dependent protein kinase termed death-associated protein kinase 2 (DAPK2) that contains an N-terminal protein kinase domain followed by a conserved CaM-binding domain with significant homologies to those of DAP kinase, a protein kinase involved in apoptosis. DAPK2 mRNA is expressed abundantly in heart, lung and skeletal muscle. The mapping results indicated that DAPK2 is located in the central region of mouse chromosome 9. In vitro kinase assay revealed that DAPK2 is autophosphorylated and phosphorylates myosin light chain (MLC) as an exogenous substrate. DAPK2 binds directly to CaM and is activated in a Ca2+/CaM-dependent manner. A constitutively active DAPK2 mutant is generated by removal of the CaM-binding domain (deltaCaM). Treatment of agonists that elevate intracellular Ca2+-concentration led to the activation of DAPK2 and transfection studies revealed that DAPK2 is localized in the cytoplasm. Overexpression of DAPK2, but not the kinase negative mutant, significantly induced the morphological changes characteristic of apoptosis. These results indicate that DAPK2 is an additional member of DAP kinase family involved in apoptotic signaling.
T Kawai; F Nomura; K Hoshino; N G Copeland; D J Gilbert; N A Jenkins; S Akira
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Oncogene     Volume:  18     ISSN:  0950-9232     ISO Abbreviation:  Oncogene     Publication Date:  1999 Jun 
Date Detail:
Created Date:  1999-06-30     Completed Date:  1999-06-30     Revised Date:  2007-11-15    
Medline Journal Info:
Nlm Unique ID:  8711562     Medline TA:  Oncogene     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  3471-80     Citation Subset:  IM    
Department of Biochemistry, Hyogo College of Medicine, Nishinomiya, Japan.
Data Bank Information
Bank Name/Acc. No.:
GENBANK/AB018001;  AB018002
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MeSH Terms
3T3 Cells
Apoptosis / physiology*
Apoptosis Regulatory Proteins
COS Cells
Calcium / metabolism
Calcium-Calmodulin-Dependent Protein Kinases / genetics,  physiology*
Calmodulin / metabolism
Chromosome Mapping
Mice, Inbred C57BL
Molecular Sequence Data
RNA, Messenger / metabolism
Tissue Distribution
Reg. No./Substance:
0/Apoptosis Regulatory Proteins; 0/Calmodulin; 0/RNA, Messenger; 7440-70-2/Calcium; EC protein kinase; EC Protein Kinases

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