Document Detail

Deamidation at Asparagine and Glutamine As a Major Modification upon Deterioration/Aging of Proteinaceous Binders in Mural Paintings.
MedLine Citation:
PMID:  21348436     Owner:  NLM     Status:  Publisher    
Proteomic strategies are herein proved to be a complementary approach to the well established amino acid composition analysis for the characterization of the aging and deterioration phenomena occurring to proteinaceous materials in works-of-art. Amino acid analyses on several samples demonstrated that proteins in the frescoes from the Camposanto Monumentale in Pisa are deteriorated as revealed by the decrease in Met, Lys, and Tyr content and by the presence in all the samples of amino malonic acid as a result of Ser, Phe, and Cys oxidation. Proteomic analysis identified deamidation at Asn and Gln as a further major event occurred. This work paves the way to the exploitation of proteomic strategies for the investigation of the molecular effects of aging and deterioration in historical objects. Results show that proteomic searches for deamidation by liquid chromatography-tandem mass spectrometry (LC-MS/MS) could constitute a routine analysis for paintings or any artistic and historic objects where proteins are present. Peptides that can be used as molecular markers when casein is present were identified.
Gabriella Leo; Ilaria Bonaduce; Alessia Andreotti; Gennaro Marino; Pietro Pucci; Maria Perla Colombini; Leila Birolo
Related Documents :
25175016 - Free fatty acid particles in protein formulations, part 1: microspectroscopic identific...
25152056 - Effect of fermentation conditions on l-lactic acid production from soybean straw hydrol...
1018016 - Preferential acid-catalyzed hydrolysis of the formamide linkage of n'-formylkynurenine ...
25263116 - Lipid characterization of individual porcine oocytes by dual mode desi-ms and data fusion.
1160206 - Oral cholecystography: osmotic activity of iopanoic glucuronide in bile.
10817286 - Effect of type of early infant feeding on fatty acid composition of plasma lipid classe...
Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2011-2-24
Journal Detail:
Title:  Analytical chemistry     Volume:  -     ISSN:  1520-6882     ISO Abbreviation:  -     Publication Date:  2011 Feb 
Date Detail:
Created Date:  2011-2-25     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0370536     Medline TA:  Anal Chem     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Dipartimento di Chimica Organica e Biochimica, Università di Napoli "Federico II" , Napoli, Italy.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Previous Document:  Dehydration-responsive reversible and irreversible changes in the extracellular matrix: comparative ...
Next Document:  Durable Hydrophilic Microchannels with Controlled Morphology by the Direct Molding Method.