Document Detail

Deae-dextran and T-cell rosette formation.
MedLine Citation:
PMID:  312247     Owner:  NLM     Status:  MEDLINE    
Use of DEAE-dextran (a polycation) increases and stabilizes the rosettes formed between sheep red cells and human peripheral blood lymphocytes. Under its influence, reproducible stable rosettes are formed after one hour of incubation in an ice bath instead of the usually required 18 to 24 hours. We have shown that rosettes are specific for T-cells and not due to "co-rosetting" of nonrosette forming cells into the T-cell rosette clusters. Only the cells from normal controls consistently show an increase in rosette formation hence routine use of this 'stabilizer' in clinical immunology is not recommended.
M Menon; S S Stefani
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Immunological communications     Volume:  8     ISSN:  0090-0877     ISO Abbreviation:  Immunol. Commun.     Publication Date:  1979  
Date Detail:
Created Date:  1979-07-28     Completed Date:  1979-07-28     Revised Date:  2004-11-17    
Medline Journal Info:
Nlm Unique ID:  0353016     Medline TA:  Immunol Commun     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  145-54     Citation Subset:  IM    
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MeSH Terms
Antilymphocyte Serum / pharmacology
DEAE-Dextran / pharmacology*
Dextrans / pharmacology*
Guinea Pigs
Middle Aged
Rosette Formation*
T-Lymphocytes / immunology*
Time Factors
Reg. No./Substance:
0/Antilymphocyte Serum; 9004-54-0/Dextrans; 9015-73-0/DEAE-Dextran

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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