| Deactivation of isoamylase and β-amylase in the agitated reactor under supercritical carbon dioxide. | |
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MedLine Citation:
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PMID: 20373113 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The current research examines the impact of agitation on deactivation of isoamylase and β-amylase under supercritical carbon dioxide (SC-CO2). Our experimental results showed that the activity of either enzyme decreased with increasing pressure or speed of agitation. The degree of enzymatic deactivation caused by pressure became more prominent in the presence of agitation, suggesting that the agitation plays an important role in enzymatic deactivation in SC-CO2 environment. Moreover, the enzymatic deactivation behavior associated with agitation and pressure was further quantitatively analyzed using a proposed inactivation kinetic model. Our analysis indicated that isoamylase and β-amylase exhibited significantly different relationships between the inverse of percentage residual activity and the product of number of revolution per time and time elapsed under pressurized carbon dioxide. We believe that the outcome from this work may provide a better understanding of the effects of agitation and pressure in enzyme deactivation behavior under SC-CO2. |
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Authors:
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Steven S-S Wang; Jinn-Tsyy Lai; Ming-Shan Huang; Clifford Y Tai; Hwai-Shen Liu |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2010-04-07 |
Journal Detail:
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Title: Bioprocess and biosystems engineering Volume: 33 ISSN: 1615-7605 ISO Abbreviation: Bioprocess Biosyst Eng Publication Date: 2010 Oct |
Date Detail:
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Created Date: 2010-09-14 Completed Date: 2011-01-13 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 101088505 Medline TA: Bioprocess Biosyst Eng Country: Germany |
Other Details:
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Languages: eng Pagination: 1007-15 Citation Subset: IM |
Affiliation:
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Department of Chemical Engineering, National Taiwan University, No. 1, Sec. 4, Roosevelt Road, Taipei, 10617, Taiwan. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Carbon Dioxide
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chemistry* Isoamylase / antagonists & inhibitors* beta-Amylase / antagonists & inhibitors* |
| Chemical | |
Reg. No./Substance:
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124-38-9/Carbon Dioxide; EC 3.2.1.2/beta-Amylase; EC 3.2.1.68/Isoamylase |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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