| De novo purine nucleotide synthesis in the rat small and large intestine: effect of dietary protein and purines. | |
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MedLine Citation:
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PMID: 6192234 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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This study assessed the pathway for de novo purine nucleotide synthesis in rat small intestinal and colonic mucosal cells, and determined the effects of dietary purines and protein on de novo purine nucleotide synthetic activity in the small intestine in vitro. Incubation of small intestinal mucosal scrapings with [14C]glycine failed to show an active pathway of de novo synthesis; in contrast, the colon showed incorporation of [14C]glycine into RNA. Rats fed a diet deficient in purines demonstrated increased incorporation of [14C]glycine into RNA-adrenine in small intestinal mucosal cells. Measurement of glutamine-amidophosphoribosyltransferase demonstrated that, regardless of the purine content of the diet, enzyme activity in the small intestine is significantly lower than in the colon or liver. The results indicate that, in the small intestine of the rat, there is an inactive de novo pathway of purine nucleotide biosynthesis that can be stimulated when purines are omitted from the diet. |
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Authors:
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N S LeLeiko; A D Bronstein; B S Baliga; H N Munro |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Journal of pediatric gastroenterology and nutrition Volume: 2 ISSN: 0277-2116 ISO Abbreviation: J. Pediatr. Gastroenterol. Nutr. Publication Date: 1983 May |
Date Detail:
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Created Date: 1983-09-23 Completed Date: 1983-09-23 Revised Date: 2003-11-14 |
Medline Journal Info:
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Nlm Unique ID: 8211545 Medline TA: J Pediatr Gastroenterol Nutr Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 313-9 Citation Subset: IM |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Amidophosphoribosyltransferase
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metabolism Animals Carbon Radioisotopes Colon / metabolism* Dietary Proteins / pharmacology* Glycine / metabolism Intestinal Mucosa / metabolism Intestine, Small / metabolism* Male Purine Nucleotides / biosynthesis* Purines / pharmacology* RNA / metabolism Rats |
| Chemical | |
Reg. No./Substance:
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0/Carbon Radioisotopes; 0/Dietary Proteins; 0/Purine Nucleotides; 0/Purines; 56-40-6/Glycine; 63231-63-0/RNA; EC 2.4.2.14/Amidophosphoribosyltransferase |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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