Document Detail

De novo fatty acid synthesis mediated by acyl-carrier protein in Neurospora crassa mitochondria.
MedLine Citation:
PMID:  2137086     Owner:  NLM     Status:  MEDLINE    
The acyl-carrier protein (ACP) in Neurospora crassa mitochondria [Brody, S. & Mikolajczyk, S. (1988) Eur. J. Biochem. 173, 353-359] mediated a cerulenin-sensitive, de novo fatty acid synthesis independent of the fatty acid synthetase complex present in the cytoplasm. Incubation of mitochondria with [2-14C]malonate labeled only the ACP as indicated by autoradiography after SDS/PAGE. Under these in vitro conditions ATP was required for the initial acyl-ACP formation, but further elongation required either magnesium or the direct addition of NADPH. Labeled hexanoic (6:0) and caprylic (8:0) acids were detected as intermediates in the pathway, as well as hydroxymyristic acid. All of the intermediates, and the eventual product of the reaction, myristic acid (14:0), were released from the ACP by alkaline treatment. Pulse-chase experiments demonstrated the incorporation on to, and release of label from, the ACP. In vivo labeling of ACP with [2-14C]malonate was also detected and the label was in the form of hydroxymyristic acid. This newly discovered pathway is discussed from the standpoint of its possible role in providing acyl chains for mitochondrial lipids.
S Mikolajczyk; S Brody
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  European journal of biochemistry / FEBS     Volume:  187     ISSN:  0014-2956     ISO Abbreviation:  Eur. J. Biochem.     Publication Date:  1990 Jan 
Date Detail:
Created Date:  1990-03-15     Completed Date:  1990-03-15     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  0107600     Medline TA:  Eur J Biochem     Country:  GERMANY, WEST    
Other Details:
Languages:  eng     Pagination:  431-7     Citation Subset:  IM    
Department of Biology, University of California, San Diego, La Jolla 92093.
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MeSH Terms
Acetyltransferases / analysis,  metabolism*
Acyl-Carrier Protein S-Acetyltransferase
Adenosine Triphosphate / pharmacology
Fatty Acids / analysis,  biosynthesis*
Magnesium / pharmacology
Malonates / metabolism*,  pharmacology
Mitochondria / drug effects,  enzymology,  metabolism*
Neurospora / metabolism*
Neurospora crassa / drug effects,  enzymology,  metabolism*
Grant Support
Reg. No./Substance:
0/Fatty Acids; 0/Malonates; 56-65-5/Adenosine Triphosphate; 7439-95-4/Magnesium; EC 2.3.1.-/Acetyltransferases; EC Protein S-Acetyltransferase

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