Document Detail

De-O-acetylation of peptidoglycan regulates glycan chain extension and affects in vivo survival of Neisseria meningitidis.
MedLine Citation:
PMID:  23373517     Owner:  NLM     Status:  Publisher    
Peptidoglycan O-acetylation is a modification found in many bacteria. In Gram-positive pathogens, it contributes to virulence by conferring resistance to host lysozyme. However, in Gram-negative pathogens, its contribution to physiology and virulence is unknown. We examined the contribution of patA, patB and ape1 to peptidoglycan O-acetylation in the major human pathogen Neisseria meningitidis (Nm). Using genetic expression of all possible combinations of the three genes in Escherichia coli and Nm, we confirmed that PatA and PatB were required for PG O-acetylation, while ApeI removed the O-acetyl group. ApeI was active on all O-acetylated muropeptides produced by PatA and PatB during heterologous expression in E. coli and was also active on several PG structures in vitro. Interestingly, in Nm, ApeI was found to preferentially de-O-acetylate muropeptides with tripeptide stems (GM3), suggesting that its activity is highly regulated. Accordingly, de-O-acetylation of GM3 regulated glycan chain elongation and cell size. Additionally, the virulence of Nm lacking ApeI was drastically reduced suggesting that regulation of glycan chain length by O-acetylation contributes to bacterial fitness in the host. Altogether, our results suggest that ApeI represents an attractive target for new drug development.
Frédéric J Veyrier; Allison H Williams; Stéphane Mesnage; Christine Schmitt; Muhamed-Kheir Taha; Ivo G Boneca
Related Documents :
22992897 - Isolation and characterization of the nematophagous fungus arthrobotrys conoides.
22938577 - Evaluation of saccharomyces cerevisiae strains as probiotic agent with aflatoxin b(1) a...
14112277 - The particulate hydrolases of macrophages. i. comparative enzymology, isolation, and pr...
23656227 - From fields to fuels: recent advances in the microbial production of biofuels.
9131467 - Identification of three lineages of wild measles virus by nucleotide sequence analysis ...
284567 - Salivary agglutinin and secretory iga reactions with oral streptococci.
Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2013-2-3
Journal Detail:
Title:  Molecular microbiology     Volume:  -     ISSN:  1365-2958     ISO Abbreviation:  Mol. Microbiol.     Publication Date:  2013 Feb 
Date Detail:
Created Date:  2013-2-4     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8712028     Medline TA:  Mol Microbiol     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Copyright Information:
© 2013 Blackwell Publishing Ltd.
Institut Pasteur, Infections Bactériennes Invasives, Département Infection et Epidémiologie, 28 Rue du Dr. Roux, Paris, F-75015, France; Institut Pasteur, Groupe Biologie et Génétique de la Paroi Bactérienne, Département de Microbiologie, 28 Rue du Dr. Roux, Paris, F-75015, France; INSERM, Groupe Avenir, Paris, F-75015, France.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Previous Document:  Effects of Sodium Hydroxide, Sodium Hypochlorite, and Gaseous Hydrogen Peroxide on the Natural Prope...
Next Document:  The crystal structure of ?-Dioxygenase provides insight into diversity in the cyclooxygenase-peroxid...